Thioclava electrotropha sp. Nov., a versatile electrode and sulfur-oxidizing bacterium from marine sediments

Rachel Chang, Lina Bird, Casey Barr, Magdalena Osburn, Elizabeth Wilbanks, Kenneth Nealson, Annette Rowe*

*Corresponding author for this work

Research output: Contribution to journalArticle

7 Scopus citations

Abstract

A taxonomic and physiologic characterization was carried out on Thioclava strain ElOx9T, which was isolated from a bacterial consortium enriched on electrodes poised at electron donating potentials. The isolate is Gram-negative, catalase-positive and oxidase-positive; the cells are motile short rods. The bacterium is facultatively anaerobic with the ability to utilize nitrate as an electron acceptor. Autotrophic growth with H2 and S0 (oxidized to sulfate) was observed. The isolate also grows heterotrophically with organic acids and sugars. Growth was observed at salinities from 0 to 10% NaCl and at temperatures from 15 to 41 °C. Phylogenetic analysis based on 16S rRNA gene sequences indicated that the strain belongs in the genus Thioclava; it had the highest sequence similarity of 98.8% to Thioclava atlantica 13D2W-2T, followed by Thioclava dalianensis DLFJ1-1T with 98.5% similarity, Thioclava pacifica TL 2T with 97.7% similarity, and then Thioclava indica DT23-4T with 96.9 %. All other sequence similarities were below 97% to characterized strains. The digital DNA–DNA hybridization estimated when compared to T. atlantica 13D2W-2T, T. dalianensis DLFJ1-1T, T. pacifica TL 2T and T. indica DT23-4T were 15.8±2.1, 16.7+2.1, 14.3±1.9 and 18.3±2.1 %. The corresponding average nucleotide identity values between these strains were determined to be 65.1, 67.8, 68.4 and 64.4 %, respectively. The G+C content of the chromosomal DNA is 63.4 mol%. Based on these results, a novel species Thioclava electrotropha sp. nov. is proposed, with the type strain ElOx9T (=DSM 103712T=ATCC TSD-100T).

Original languageEnglish (US)
Article number002723
Pages (from-to)1652-1658
Number of pages7
JournalInternational Journal of Systematic and Evolutionary Microbiology
Volume68
Issue number5
DOIs
StatePublished - May 2018

Keywords

  • Cathode oxidation
  • Chemolithoautotroph
  • Marine sediment
  • Sulfur oxidation

ASJC Scopus subject areas

  • Microbiology
  • Ecology, Evolution, Behavior and Systematics

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