Thiophosphate interference experiments locate phosphates important for the hammerhead RNA self-cleavage reaction

Duane E. Ruffner, Olke C. Uhlenbeck*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

146 Scopus citations

Abstract

A hammerhead domain of less than 50 nucleotides is responsible for a self-cleavage reaction in the replication of plant RNA pathogens. The hammerhead is composed of three helices joining at a central conserved core of 11 single stranded nucleotides. The core is believed to fold into a tertiary structure that provides functional groups for catalysis and to coordinate one or more divalent metal ions. In this study we use a phosphorothioate substitution interference assay to identify four phosphates in the conserved core which also play a role in the self-cleavage reaction.

Original languageEnglish (US)
Pages (from-to)6025-6029
Number of pages5
JournalNucleic acids research
Volume18
Issue number20
DOIs
StatePublished - Oct 25 1990

ASJC Scopus subject areas

  • Genetics

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