Thiophosphate interference experiments locate phosphates important for the hammerhead RNA self-cleavage reaction

Duane E. Ruffner; Olke C. Uhlenbeck

doi:10.1093/nar/18.20.6025

Thiophosphate interference experiments locate phosphates important for the hammerhead RNA self-cleavage reaction

Duane E. Ruffner, Olke C. Uhlenbeck^*

^*Corresponding author for this work

Molecular Biosciences

Research output: Contribution to journal › Article › peer-review

158 Scopus citations

Abstract

A hammerhead domain of less than 50 nucleotides is responsible for a self-cleavage reaction in the replication of plant RNA pathogens. The hammerhead is composed of three helices joining at a central conserved core of 11 single stranded nucleotides. The core is believed to fold into a tertiary structure that provides functional groups for catalysis and to coordinate one or more divalent metal ions. In this study we use a phosphorothioate substitution interference assay to identify four phosphates in the conserved core which also play a role in the self-cleavage reaction.

Original language	English (US)
Pages (from-to)	6025-6029
Number of pages	5
Journal	Nucleic acids research
Volume	18
Issue number	20
DOIs	https://doi.org/10.1093/nar/18.20.6025
State	Published - Oct 25 1990

Funding

We thank Fritz Eckstein for his generous gift of NTPaS. DER and OCU were supported by NIH grants GM36944 and AI30242. We also thank the W.M.Keck Foundation for their generous support of RNA science on the Boulder campus.

ASJC Scopus subject areas

Genetics

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abstract = "A hammerhead domain of less than 50 nucleotides is responsible for a self-cleavage reaction in the replication of plant RNA pathogens. The hammerhead is composed of three helices joining at a central conserved core of 11 single stranded nucleotides. The core is believed to fold into a tertiary structure that provides functional groups for catalysis and to coordinate one or more divalent metal ions. In this study we use a phosphorothioate substitution interference assay to identify four phosphates in the conserved core which also play a role in the self-cleavage reaction.",

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note = "Funding Information: We thank Fritz Eckstein for his generous gift of NTPaS. DER and OCU were supported by NIH grants GM36944 and AI30242. We also thank the W.M.Keck Foundation for their generous support of RNA science on the Boulder campus.",

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Thiophosphate interference experiments locate phosphates important for the hammerhead RNA self-cleavage reaction

Abstract

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