Three-dimensional imaging of skin melanoma in vivo by dual-wavelength photoacoustic microscopy

Jung Taek Oh; Meng Lin Li; Hao F. Zhang; Konstantin Maslov; George Stoica; Lihong V. Wang

doi:10.1117/1.2210907

Three-dimensional imaging of skin melanoma in vivo by dual-wavelength photoacoustic microscopy

Jung Taek Oh, Meng Lin Li, Hao F. Zhang, Konstantin Maslov, George Stoica, Lihong V. Wang^*

^*Corresponding author for this work

Biomedical Engineering

Research output: Contribution to journal › Article › peer-review

276 Scopus citations

Abstract

Dual-wavelength reflection-mode photoacoustic microscopy is used to noninvasively obtain three-dimensional (3-D) images of subcutaneous melanomas and their surrounding vasculature in nude mice in vivo. The absorption coefficients of blood and melaninpigmented melanomas vary greatly relative to each other at these two optical wavelengths (764 and 584 nm). Using high-resolution and high-contrast photoacoustic imaging in vivo with a near-infrared (764-nm) light source, the 3-D melanin distribution inside the skin is imaged, and the maximum thickness of the melanoma (∼0.5 mm) is measured. The vascular system surrounding the melanoma is also imaged with visible light (584 nm) and the tumor-feeding vessels found. This technique can potentially be used for melanoma diagnosis, prognosis, and treatment planning.

Original language	English (US)
Article number	034032
Journal	Journal of Biomedical Optics
Volume	11
Issue number	3
DOIs	https://doi.org/10.1117/1.2210907
State	Published - May 2006

Funding

This research was supported in part by the National Institutes of Health grants R01 EB000712 and R01 NS46214. J. T. Oh was supported in part by the postdoctoral Fellowship Program of Korea Science & Engineering Foundation (KOSEF).

Keywords

Medical and biological imaging
Photoacoustic imaging
Spectroscopy
Three-dimensional microscopy
Tissue diagnostics

ASJC Scopus subject areas

Electronic, Optical and Magnetic Materials
Atomic and Molecular Physics, and Optics
Biomedical Engineering
Biomaterials

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1117/1.2210907

Cite this

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title = "Three-dimensional imaging of skin melanoma in vivo by dual-wavelength photoacoustic microscopy",

abstract = "Dual-wavelength reflection-mode photoacoustic microscopy is used to noninvasively obtain three-dimensional (3-D) images of subcutaneous melanomas and their surrounding vasculature in nude mice in vivo. The absorption coefficients of blood and melaninpigmented melanomas vary greatly relative to each other at these two optical wavelengths (764 and 584 nm). Using high-resolution and high-contrast photoacoustic imaging in vivo with a near-infrared (764-nm) light source, the 3-D melanin distribution inside the skin is imaged, and the maximum thickness of the melanoma (∼0.5 mm) is measured. The vascular system surrounding the melanoma is also imaged with visible light (584 nm) and the tumor-feeding vessels found. This technique can potentially be used for melanoma diagnosis, prognosis, and treatment planning.",

keywords = "Medical and biological imaging, Photoacoustic imaging, Spectroscopy, Three-dimensional microscopy, Tissue diagnostics",

author = "Oh, {Jung Taek} and Li, {Meng Lin} and Zhang, {Hao F.} and Konstantin Maslov and George Stoica and Wang, {Lihong V.}",

note = "Funding Information: This research was supported in part by the National Institutes of Health grants R01 EB000712 and R01 NS46214. J. T. Oh was supported in part by the postdoctoral Fellowship Program of Korea Science & Engineering Foundation (KOSEF).",

year = "2006",

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doi = "10.1117/1.2210907",

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volume = "11",

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AU - Oh, Jung Taek

AU - Li, Meng Lin

AU - Zhang, Hao F.

AU - Maslov, Konstantin

AU - Stoica, George

AU - Wang, Lihong V.

N1 - Funding Information: This research was supported in part by the National Institutes of Health grants R01 EB000712 and R01 NS46214. J. T. Oh was supported in part by the postdoctoral Fellowship Program of Korea Science & Engineering Foundation (KOSEF).

PY - 2006/5

Y1 - 2006/5

N2 - Dual-wavelength reflection-mode photoacoustic microscopy is used to noninvasively obtain three-dimensional (3-D) images of subcutaneous melanomas and their surrounding vasculature in nude mice in vivo. The absorption coefficients of blood and melaninpigmented melanomas vary greatly relative to each other at these two optical wavelengths (764 and 584 nm). Using high-resolution and high-contrast photoacoustic imaging in vivo with a near-infrared (764-nm) light source, the 3-D melanin distribution inside the skin is imaged, and the maximum thickness of the melanoma (∼0.5 mm) is measured. The vascular system surrounding the melanoma is also imaged with visible light (584 nm) and the tumor-feeding vessels found. This technique can potentially be used for melanoma diagnosis, prognosis, and treatment planning.

AB - Dual-wavelength reflection-mode photoacoustic microscopy is used to noninvasively obtain three-dimensional (3-D) images of subcutaneous melanomas and their surrounding vasculature in nude mice in vivo. The absorption coefficients of blood and melaninpigmented melanomas vary greatly relative to each other at these two optical wavelengths (764 and 584 nm). Using high-resolution and high-contrast photoacoustic imaging in vivo with a near-infrared (764-nm) light source, the 3-D melanin distribution inside the skin is imaged, and the maximum thickness of the melanoma (∼0.5 mm) is measured. The vascular system surrounding the melanoma is also imaged with visible light (584 nm) and the tumor-feeding vessels found. This technique can potentially be used for melanoma diagnosis, prognosis, and treatment planning.

KW - Medical and biological imaging

KW - Photoacoustic imaging

KW - Spectroscopy

KW - Three-dimensional microscopy

KW - Tissue diagnostics

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Three-dimensional imaging of skin melanoma in vivo by dual-wavelength photoacoustic microscopy

Abstract

Funding

Keywords

ASJC Scopus subject areas

UN SDGs

Access to Document

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