Three-dimensional representation and cortical projection topography of the nucleus basalis (Ch4) in the macaque: concurrent demonstration of choline acetyltransferase and retrograde transport with a stabilized tetramethylbenzidine method for horseradish peroxidase

M. Marsel Mesulam; Elliott J. Mufson; Bruce H. Wainer

doi:10.1016/0006-8993(86)91607-0

Three-dimensional representation and cortical projection topography of the nucleus basalis (Ch4) in the macaque: concurrent demonstration of choline acetyltransferase and retrograde transport with a stabilized tetramethylbenzidine method for horseradish peroxidase

M. Marsel Mesulam^*, Elliott J. Mufson, Bruce H. Wainer

^*Corresponding author for this work

Neurology

Research output: Contribution to journal › Article › peer-review

92 Scopus citations

Abstract

Ninety-six percent of the nucleus basalis neurons that project to the neocortex contain choline acetyltransferase. These projections from the cholinergic component of the nucleus basalis (Ch4) are topographically organized so that each cortical area receives most of its cholinergic input from a different Ch4 sector. The three-dimensional reconstruction of these sectors reveals the presence of a complex structure. A stabilization procedure that was used in these experiments maintains all the advantages of the tetramethylbenzidine method for horseradish peroxidase while eliminating the vulnerability of the reaction-product to high pH and dehydrating agents.

Original language	English (US)
Pages (from-to)	301-308
Number of pages	8
Journal	Brain research
Volume	367
Issue number	1-2
DOIs	https://doi.org/10.1016/0006-8993(86)91607-0
State	Published - Mar 5 1986

Funding

We want to thank Leah Christie, Terry Martin, Rick Plourde and Marcia Williams for expert secretarial, technical and artistic assistance. We also want to thank Dr. D.B. Rye who helped us with the histochemical and immunohistochemical preparations. Supported in part by the ADRDA Faculty Scholar Award (to E.J.M.), the Javits Neuroscience Investigator Award; the McKnight Foundation, an Alzheimer's Research Center NIA AG05134, NS-17661 and HD-04583.

Keywords

choline acetyltransferase
cholinergic projection
horseradish peroxidase
nucleus basalis
tetramethylbenzidine

ASJC Scopus subject areas

General Neuroscience
Molecular Biology
Clinical Neurology
Developmental Biology

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Mesulam, M. M., Mufson, E. J., & Wainer, B. H. (1986). Three-dimensional representation and cortical projection topography of the nucleus basalis (Ch4) in the macaque: concurrent demonstration of choline acetyltransferase and retrograde transport with a stabilized tetramethylbenzidine method for horseradish peroxidase. Brain research, 367(1-2), 301-308. https://doi.org/10.1016/0006-8993(86)91607-0

Mesulam, M. Marsel ; Mufson, Elliott J. ; Wainer, Bruce H. / Three-dimensional representation and cortical projection topography of the nucleus basalis (Ch4) in the macaque : concurrent demonstration of choline acetyltransferase and retrograde transport with a stabilized tetramethylbenzidine method for horseradish peroxidase. In: Brain research. 1986 ; Vol. 367, No. 1-2. pp. 301-308.

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title = "Three-dimensional representation and cortical projection topography of the nucleus basalis (Ch4) in the macaque: concurrent demonstration of choline acetyltransferase and retrograde transport with a stabilized tetramethylbenzidine method for horseradish peroxidase",

abstract = "Ninety-six percent of the nucleus basalis neurons that project to the neocortex contain choline acetyltransferase. These projections from the cholinergic component of the nucleus basalis (Ch4) are topographically organized so that each cortical area receives most of its cholinergic input from a different Ch4 sector. The three-dimensional reconstruction of these sectors reveals the presence of a complex structure. A stabilization procedure that was used in these experiments maintains all the advantages of the tetramethylbenzidine method for horseradish peroxidase while eliminating the vulnerability of the reaction-product to high pH and dehydrating agents.",

keywords = "choline acetyltransferase, cholinergic projection, horseradish peroxidase, nucleus basalis, tetramethylbenzidine",

author = "Mesulam, {M. Marsel} and Mufson, {Elliott J.} and Wainer, {Bruce H.}",

note = "Funding Information: We want to thank Leah Christie, Terry Martin, Rick Plourde and Marcia Williams for expert secretarial, technical and artistic assistance. We also want to thank Dr. D.B. Rye who helped us with the histochemical and immunohistochemical preparations. Supported in part by the ADRDA Faculty Scholar Award (to E.J.M.), the Javits Neuroscience Investigator Award; the McKnight Foundation, an Alzheimer's Research Center NIA AG05134, NS-17661 and HD-04583.",

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Mesulam, MM, Mufson, EJ & Wainer, BH 1986, 'Three-dimensional representation and cortical projection topography of the nucleus basalis (Ch4) in the macaque: concurrent demonstration of choline acetyltransferase and retrograde transport with a stabilized tetramethylbenzidine method for horseradish peroxidase', Brain research, vol. 367, no. 1-2, pp. 301-308. https://doi.org/10.1016/0006-8993(86)91607-0

Three-dimensional representation and cortical projection topography of the nucleus basalis (Ch4) in the macaque: concurrent demonstration of choline acetyltransferase and retrograde transport with a stabilized tetramethylbenzidine method for horseradish peroxidase. / Mesulam, M. Marsel; Mufson, Elliott J.; Wainer, Bruce H.
In: Brain research, Vol. 367, No. 1-2, 05.03.1986, p. 301-308.

Research output: Contribution to journal › Article › peer-review

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AU - Mesulam, M. Marsel

AU - Mufson, Elliott J.

AU - Wainer, Bruce H.

N1 - Funding Information: We want to thank Leah Christie, Terry Martin, Rick Plourde and Marcia Williams for expert secretarial, technical and artistic assistance. We also want to thank Dr. D.B. Rye who helped us with the histochemical and immunohistochemical preparations. Supported in part by the ADRDA Faculty Scholar Award (to E.J.M.), the Javits Neuroscience Investigator Award; the McKnight Foundation, an Alzheimer's Research Center NIA AG05134, NS-17661 and HD-04583.

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N2 - Ninety-six percent of the nucleus basalis neurons that project to the neocortex contain choline acetyltransferase. These projections from the cholinergic component of the nucleus basalis (Ch4) are topographically organized so that each cortical area receives most of its cholinergic input from a different Ch4 sector. The three-dimensional reconstruction of these sectors reveals the presence of a complex structure. A stabilization procedure that was used in these experiments maintains all the advantages of the tetramethylbenzidine method for horseradish peroxidase while eliminating the vulnerability of the reaction-product to high pH and dehydrating agents.

AB - Ninety-six percent of the nucleus basalis neurons that project to the neocortex contain choline acetyltransferase. These projections from the cholinergic component of the nucleus basalis (Ch4) are topographically organized so that each cortical area receives most of its cholinergic input from a different Ch4 sector. The three-dimensional reconstruction of these sectors reveals the presence of a complex structure. A stabilization procedure that was used in these experiments maintains all the advantages of the tetramethylbenzidine method for horseradish peroxidase while eliminating the vulnerability of the reaction-product to high pH and dehydrating agents.

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Mesulam MM, Mufson EJ, Wainer BH. Three-dimensional representation and cortical projection topography of the nucleus basalis (Ch4) in the macaque: concurrent demonstration of choline acetyltransferase and retrograde transport with a stabilized tetramethylbenzidine method for horseradish peroxidase. Brain research. 1986 Mar 5;367(1-2):301-308. doi: 10.1016/0006-8993(86)91607-0

Three-dimensional representation and cortical projection topography of the nucleus basalis (Ch4) in the macaque: concurrent demonstration of choline acetyltransferase and retrograde transport with a stabilized tetramethylbenzidine method for horseradish peroxidase

Abstract

Funding

Keywords

ASJC Scopus subject areas

Access to Document

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