Three-dimensional, sharp-tipped electrodes concentrate applied fields to enable direct electrical release of intact biomarkers from cells

Mahla Poudineh, Reza M. Mohamadi, Andrew Sage, Laili Mahmoudian, Edward H. Sargent*, Shana O. Kelley

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

23 Scopus citations

Abstract

Biomarkers such as proteins and nucleic acids released from human cells, bacteria, and viruses offer a wealth of information pertinent to diagnosis and treatment ranging from cancer to infectious disease. The release of these molecules from within cells is a crucial step in biomarker analysis. Here we show that purely electric-field-driven lysis can be achieved, inline, within a microfluidic channel; that it can produce highly efficient lysis and biomarker release; and, further, that it can do so with minimal degradation of the released biomarkers. Central to this new technology is the use of three-dimensional sharp-tipped electrodes (3DSTEs) in lysis, which we prove using experiment and finite-element modeling produce the electric field concentration necessary for efficient cell wall rupture.

Original languageEnglish (US)
Pages (from-to)1785-1790
Number of pages6
JournalLab on a Chip
Volume14
Issue number10
DOIs
StatePublished - 2014

ASJC Scopus subject areas

  • General Chemistry
  • Bioengineering
  • Biochemistry
  • Biomedical Engineering

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