Three-dimensional structure of a recombinant gap junction membrane channel

Vinzenz M. Unger, Nalin M. Kumar, Norton B. Gilula, Mark Yeager*

*Corresponding author for this work

Research output: Contribution to journalArticle

424 Scopus citations

Abstract

Gap junction membrane channels mediate electrical and metabolic coupling between adjacent cells. The structure of a recombinant cardiac gap junction channel was determined by electron crystallography at resolutions of 7.5 angstroms in the membrane plane and 21 angstroms in the vertical direction. The dodecameric channel was formed by the end-to-end docking of two hexamers, each of which displayed 24 rods of density in the membrane interior, which is consistent with an α-helical conformation for the four transmembrane domains of each connexin subunit. The transmembrane α-helical rods contrasted with the double-layered appearance of the extracellular domains. Although not indicative for a particular type of secondary structure, the protein density that formed the extracellular vestibule provided a tight seal to exclude the exchange of substances with the extracellular milieu.

Original languageEnglish (US)
Pages (from-to)1176-1180
Number of pages5
JournalScience
Volume283
Issue number5405
DOIs
StatePublished - Feb 19 1999

ASJC Scopus subject areas

  • General

Fingerprint Dive into the research topics of 'Three-dimensional structure of a recombinant gap junction membrane channel'. Together they form a unique fingerprint.

Cite this