TY - JOUR
T1 - Three distinct antigens associated with human T-lymphocyte-mediated cytolysis
T2 - LFA-1, LFA-2, and LFA-3
AU - Sanchez-Madrid, F.
AU - Krensky, A. M.
AU - Ware, C. F.
AU - Robbins, E.
AU - Strominger, J. L.
AU - Burakoff, S. J.
AU - Springer, T. A.
PY - 1982
Y1 - 1982
N2 - Monoclonal antibodies were prepared to anti-HLA-DR cytolytic T lymphocytes (CTLs) and screened for inhibition of CTL-mediated killing. Binding of monoclonal antibodies to four types of molecules, LFA-1, LFA-2, LFA-3, and HLA-DR, inhibited killing, suggesting that these molecules participate in the CTL-target cell interaction. The antigens were characterized by immunoprecipitation, crosslinking, NaDodSO 4/polyacrylamide gel electrophoresis, and immunofluorescence flow cytometry. The LFA-1 antigen contains α and β polypeptide chains of M(r) 177,000 and 95,000 that are noncovalently associated in an α 1β 1 structure. It is present on both B and T lymphocytes and marks subpopulations that differ in quantitative expression. Human LFA-1 appears to be the homologue of mouse LFA-1. Human LFA-2 is of M(r) 49,000 with a minor component of M(r) 36,000. It is expressed on CTL lines but not on a B-cell line and in peripheral blood preferentially on T lymphocytes. Human LFA-3 is of M(r) 60,000 and is expressed on both B and T lymphocytes.
AB - Monoclonal antibodies were prepared to anti-HLA-DR cytolytic T lymphocytes (CTLs) and screened for inhibition of CTL-mediated killing. Binding of monoclonal antibodies to four types of molecules, LFA-1, LFA-2, LFA-3, and HLA-DR, inhibited killing, suggesting that these molecules participate in the CTL-target cell interaction. The antigens were characterized by immunoprecipitation, crosslinking, NaDodSO 4/polyacrylamide gel electrophoresis, and immunofluorescence flow cytometry. The LFA-1 antigen contains α and β polypeptide chains of M(r) 177,000 and 95,000 that are noncovalently associated in an α 1β 1 structure. It is present on both B and T lymphocytes and marks subpopulations that differ in quantitative expression. Human LFA-1 appears to be the homologue of mouse LFA-1. Human LFA-2 is of M(r) 49,000 with a minor component of M(r) 36,000. It is expressed on CTL lines but not on a B-cell line and in peripheral blood preferentially on T lymphocytes. Human LFA-3 is of M(r) 60,000 and is expressed on both B and T lymphocytes.
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U2 - 10.1073/pnas.79.23.7489
DO - 10.1073/pnas.79.23.7489
M3 - Article
C2 - 6984191
AN - SCOPUS:3042997577
SN - 0027-8424
VL - 79
SP - 7489
EP - 7493
JO - Proceedings of the National Academy of Sciences of the United States of America
JF - Proceedings of the National Academy of Sciences of the United States of America
IS - 23 I
ER -