Three-layer composite magnetic nanoparticle probes for DNA

Savka I. Stoeva; Fengwei Huo; Jae Seung Lee; Chad A. Mirkin

doi:10.1021/ja055056d

Three-layer composite magnetic nanoparticle probes for DNA

Savka I. Stoeva, Fengwei Huo, Jae Seung Lee, Chad A. Mirkin^*

^*Corresponding author for this work

Chemistry

Research output: Contribution to journal › Article › peer-review

299 Scopus citations

Abstract

A method for synthesizing composite nanoparticles with a gold shell, an Fe₃O₄ inner shell, and a silica core has been developed. The approach utilizes positively charged amino-modified SiO₂ particles as templates for the assembly of negatively charged 15 nm superparamagnetic water-soluble Fe₃O₄ nanoparticles. The SiO₂-Fe₃O₄ particles electrostatically attract 1-3 nm Au nanoparticle seeds that act in a subsequent step as nucleation sites for the formation of a continuous gold shell around the SiO₂-Fe₃O₄ particles upon HAuCl₄ reduction. The three-layer magnetic nanoparticles, when functionalized with oligonucleotides, exhibit the surface chemistry, optical properties, and cooperative DNA binding properties of gold nanoparticle probes, but the magnetic properties of the Fe₃O₄ inner shell.

Original language	English (US)
Pages (from-to)	15362-15363
Number of pages	2
Journal	Journal of the American Chemical Society
Volume	127
Issue number	44
DOIs	https://doi.org/10.1021/ja055056d
State	Published - Nov 9 2005

ASJC Scopus subject areas

Catalysis
General Chemistry
Biochemistry
Colloid and Surface Chemistry

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abstract = "A method for synthesizing composite nanoparticles with a gold shell, an Fe3O4 inner shell, and a silica core has been developed. The approach utilizes positively charged amino-modified SiO2 particles as templates for the assembly of negatively charged 15 nm superparamagnetic water-soluble Fe3O4 nanoparticles. The SiO2-Fe3O4 particles electrostatically attract 1-3 nm Au nanoparticle seeds that act in a subsequent step as nucleation sites for the formation of a continuous gold shell around the SiO2-Fe3O4 particles upon HAuCl4 reduction. The three-layer magnetic nanoparticles, when functionalized with oligonucleotides, exhibit the surface chemistry, optical properties, and cooperative DNA binding properties of gold nanoparticle probes, but the magnetic properties of the Fe3O4 inner shell.",

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AU - Stoeva, Savka I.

AU - Huo, Fengwei

AU - Lee, Jae Seung

AU - Mirkin, Chad A.

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AB - A method for synthesizing composite nanoparticles with a gold shell, an Fe3O4 inner shell, and a silica core has been developed. The approach utilizes positively charged amino-modified SiO2 particles as templates for the assembly of negatively charged 15 nm superparamagnetic water-soluble Fe3O4 nanoparticles. The SiO2-Fe3O4 particles electrostatically attract 1-3 nm Au nanoparticle seeds that act in a subsequent step as nucleation sites for the formation of a continuous gold shell around the SiO2-Fe3O4 particles upon HAuCl4 reduction. The three-layer magnetic nanoparticles, when functionalized with oligonucleotides, exhibit the surface chemistry, optical properties, and cooperative DNA binding properties of gold nanoparticle probes, but the magnetic properties of the Fe3O4 inner shell.

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Three-layer composite magnetic nanoparticle probes for DNA

Abstract

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