Thy-1 inhibits mitogen-induced Ca2+ oscillation in ras-transformed mouse fibroblasts

Yoshikazu Sugimoto*, Tao Fu, Rei Hirochika, Hiromitsu Nakauchi, Yoji Ikawa, Yoshinori Nozawa

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

4 Scopus citations

Abstract

Cell surface glycoprotein Thy-1 functions as a transformation suppressor in v-ras-transformed NIH/3T3 cells [Sugimoto et al., (1991) Cancer Res. 51, 99-104.]. In order to understand the mechanism of action of Thy-1, we examined the effect of Thy-1 expression on mitogen-induced Ca2+ oscillation which was correlated with v-ras-transformation [Fu et al., (1991) FEBS Lett. 281, 263-266.]. Forced expression of Thy-1 in v-ras-transformed cells inhibited mitogen-induced Ca2+ oscillation. Although v-Ras-free, Thy-1-positive NIH/3T3 cells (major population) did not show Ca2+ oscillation, whereas in Thy-1-negative NIH/3T3 cells (less than 1% of the population) Ca2+ oscillation was observed. Finally, replacement of the carboxyl-half of Thy-1 with that of CD4 abolished the inhibitory effect of Thy-1. These results suggest that Thy-1 directly or indirectly participates in the negative regulation of Ca2+ response by inhibiting Ca2+ oscillation.

Original languageEnglish (US)
Pages (from-to)230-235
Number of pages6
JournalExperimental Cell Research
Volume203
Issue number1
DOIs
StatePublished - Nov 1992

ASJC Scopus subject areas

  • Cell Biology

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