Thy-1 inhibits mitogen-induced Ca2+ oscillation in ras-transformed mouse fibroblasts

Yoshikazu Sugimoto*, Tao Fu, Rei Hirochika, Hiromitsu Nakauchi, Yoji Ikawa, Yoshinori Nozawa

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

5 Scopus citations

Abstract

Cell surface glycoprotein Thy-1 functions as a transformation suppressor in v-ras-transformed NIH/3T3 cells [Sugimoto et al., (1991) Cancer Res. 51, 99-104.]. In order to understand the mechanism of action of Thy-1, we examined the effect of Thy-1 expression on mitogen-induced Ca2+ oscillation which was correlated with v-ras-transformation [Fu et al., (1991) FEBS Lett. 281, 263-266.]. Forced expression of Thy-1 in v-ras-transformed cells inhibited mitogen-induced Ca2+ oscillation. Although v-Ras-free, Thy-1-positive NIH/3T3 cells (major population) did not show Ca2+ oscillation, whereas in Thy-1-negative NIH/3T3 cells (less than 1% of the population) Ca2+ oscillation was observed. Finally, replacement of the carboxyl-half of Thy-1 with that of CD4 abolished the inhibitory effect of Thy-1. These results suggest that Thy-1 directly or indirectly participates in the negative regulation of Ca2+ response by inhibiting Ca2+ oscillation.

Original languageEnglish (US)
Pages (from-to)230-235
Number of pages6
JournalExperimental Cell Research
Volume203
Issue number1
DOIs
StatePublished - Nov 1992

Funding

We thank Shigeko Murakami for cell cultures, Miu Isoda and Ka-zunari Nagayoshi for FACS operation, and Roger Morris and Renu Wadhwa for critical reading of the manuscript. This work was supported in part by grants from the Agency of Science and Technology (Y.S. and H.N.) and the Ministry of Education, Science and Culture of Japan (Y.N.).

ASJC Scopus subject areas

  • Cell Biology

Fingerprint

Dive into the research topics of 'Thy-1 inhibits mitogen-induced Ca2+ oscillation in ras-transformed mouse fibroblasts'. Together they form a unique fingerprint.

Cite this