Abstract
Cell surface glycoprotein Thy-1 functions as a transformation suppressor in v-ras-transformed NIH/3T3 cells [Sugimoto et al., (1991) Cancer Res. 51, 99-104.]. In order to understand the mechanism of action of Thy-1, we examined the effect of Thy-1 expression on mitogen-induced Ca2+ oscillation which was correlated with v-ras-transformation [Fu et al., (1991) FEBS Lett. 281, 263-266.]. Forced expression of Thy-1 in v-ras-transformed cells inhibited mitogen-induced Ca2+ oscillation. Although v-Ras-free, Thy-1-positive NIH/3T3 cells (major population) did not show Ca2+ oscillation, whereas in Thy-1-negative NIH/3T3 cells (less than 1% of the population) Ca2+ oscillation was observed. Finally, replacement of the carboxyl-half of Thy-1 with that of CD4 abolished the inhibitory effect of Thy-1. These results suggest that Thy-1 directly or indirectly participates in the negative regulation of Ca2+ response by inhibiting Ca2+ oscillation.
Original language | English (US) |
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Pages (from-to) | 230-235 |
Number of pages | 6 |
Journal | Experimental Cell Research |
Volume | 203 |
Issue number | 1 |
DOIs | |
State | Published - Nov 1992 |
Funding
We thank Shigeko Murakami for cell cultures, Miu Isoda and Ka-zunari Nagayoshi for FACS operation, and Roger Morris and Renu Wadhwa for critical reading of the manuscript. This work was supported in part by grants from the Agency of Science and Technology (Y.S. and H.N.) and the Ministry of Education, Science and Culture of Japan (Y.N.).
ASJC Scopus subject areas
- Cell Biology