Thyroid-specific and hormone-dependent expression of rat thyroglobulin promoter fused with bacterial chloramphenicol acetyltransferase gene in transgenic mice

Cheryl A. Skinner, Donna Kelly, Ling Rong Kao, Shihadeh N. Nayfeh, Brian Popko, Chi Bom Chae*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

6 Scopus citations

Abstract

The minimal promoter of rat thyroglobulin (TG) gene (168 bp) was fused with bacterial chloramphenicol acetyltransferase (CAT) gene, and transgenic mice carrying the TGCAT gene were produced. The minimal promoter is sufficient for thyroid-specific and hormone-dependent expression of TGCAT in transgenic mice. Deletion of a region between -128 and -92 bp (TGII), which is not required for the expression of TGCAT in transient expression assays but whose sequence is most extensively conserved among different species, appears to decrease frequency of the expression of TGCAT in transgenic mice. However, the same deletion apparently has no significant effect on TG promoter activity in stably transformed rat FRTL-5 cells.

Original languageEnglish (US)
Pages (from-to)33-38
Number of pages6
JournalMolecular and Cellular Endocrinology
Volume90
Issue number1
DOIs
StatePublished - Dec 1992

Keywords

  • Gene expression
  • Minimal promoter
  • Thyroglobulin gene
  • Thyrotropin
  • Transgenic mice

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Endocrinology

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