Thyroid-specific and hormone-dependent expression of rat thyroglobulin promoter fused with bacterial chloramphenicol acetyltransferase gene in transgenic mice

Cheryl A. Skinner; Donna Kelly; Ling Rong Kao; Shihadeh N. Nayfeh; Brian Popko; Chi Bom Chae

doi:10.1016/0303-7207(92)90098-Q

Thyroid-specific and hormone-dependent expression of rat thyroglobulin promoter fused with bacterial chloramphenicol acetyltransferase gene in transgenic mice

Cheryl A. Skinner, Donna Kelly, Ling Rong Kao, Shihadeh N. Nayfeh, Brian Popko, Chi Bom Chae^*

^*Corresponding author for this work

Neurology

Research output: Contribution to journal › Article › peer-review

6 Scopus citations

Abstract

The minimal promoter of rat thyroglobulin (TG) gene (168 bp) was fused with bacterial chloramphenicol acetyltransferase (CAT) gene, and transgenic mice carrying the TGCAT gene were produced. The minimal promoter is sufficient for thyroid-specific and hormone-dependent expression of TGCAT in transgenic mice. Deletion of a region between -128 and -92 bp (TGII), which is not required for the expression of TGCAT in transient expression assays but whose sequence is most extensively conserved among different species, appears to decrease frequency of the expression of TGCAT in transgenic mice. However, the same deletion apparently has no significant effect on TG promoter activity in stably transformed rat FRTL-5 cells.

Original language	English (US)
Pages (from-to)	33-38
Number of pages	6
Journal	Molecular and Cellular Endocrinology
Volume	90
Issue number	1
DOIs	https://doi.org/10.1016/0303-7207(92)90098-Q
State	Published - Dec 1992

Funding

Keywords

Gene expression
Minimal promoter
Thyroglobulin gene
Thyrotropin
Transgenic mice

ASJC Scopus subject areas

Endocrinology
Molecular Biology
Biochemistry

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10.1016/0303-7207(92)90098-Q

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title = "Thyroid-specific and hormone-dependent expression of rat thyroglobulin promoter fused with bacterial chloramphenicol acetyltransferase gene in transgenic mice",

abstract = "The minimal promoter of rat thyroglobulin (TG) gene (168 bp) was fused with bacterial chloramphenicol acetyltransferase (CAT) gene, and transgenic mice carrying the TGCAT gene were produced. The minimal promoter is sufficient for thyroid-specific and hormone-dependent expression of TGCAT in transgenic mice. Deletion of a region between -128 and -92 bp (TGII), which is not required for the expression of TGCAT in transient expression assays but whose sequence is most extensively conserved among different species, appears to decrease frequency of the expression of TGCAT in transgenic mice. However, the same deletion apparently has no significant effect on TG promoter activity in stably transformed rat FRTL-5 cells.",

keywords = "Gene expression, Minimal promoter, Thyroglobulin gene, Thyrotropin, Transgenic mice",

author = "Skinner, {Cheryl A.} and Donna Kelly and Kao, {Ling Rong} and Nayfeh, {Shihadeh N.} and Brian Popko and Chae, {Chi Bom}",

year = "1992",

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AU - Skinner, Cheryl A.

AU - Kelly, Donna

AU - Kao, Ling Rong

AU - Nayfeh, Shihadeh N.

AU - Popko, Brian

AU - Chae, Chi Bom

PY - 1992/12

Y1 - 1992/12

N2 - The minimal promoter of rat thyroglobulin (TG) gene (168 bp) was fused with bacterial chloramphenicol acetyltransferase (CAT) gene, and transgenic mice carrying the TGCAT gene were produced. The minimal promoter is sufficient for thyroid-specific and hormone-dependent expression of TGCAT in transgenic mice. Deletion of a region between -128 and -92 bp (TGII), which is not required for the expression of TGCAT in transient expression assays but whose sequence is most extensively conserved among different species, appears to decrease frequency of the expression of TGCAT in transgenic mice. However, the same deletion apparently has no significant effect on TG promoter activity in stably transformed rat FRTL-5 cells.

AB - The minimal promoter of rat thyroglobulin (TG) gene (168 bp) was fused with bacterial chloramphenicol acetyltransferase (CAT) gene, and transgenic mice carrying the TGCAT gene were produced. The minimal promoter is sufficient for thyroid-specific and hormone-dependent expression of TGCAT in transgenic mice. Deletion of a region between -128 and -92 bp (TGII), which is not required for the expression of TGCAT in transient expression assays but whose sequence is most extensively conserved among different species, appears to decrease frequency of the expression of TGCAT in transgenic mice. However, the same deletion apparently has no significant effect on TG promoter activity in stably transformed rat FRTL-5 cells.

KW - Gene expression

KW - Minimal promoter

KW - Thyroglobulin gene

KW - Thyrotropin

KW - Transgenic mice

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Thyroid-specific and hormone-dependent expression of rat thyroglobulin promoter fused with bacterial chloramphenicol acetyltransferase gene in transgenic mice

Abstract

Funding

Keywords

ASJC Scopus subject areas

Access to Document

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