Tilt-invariant scanned oblique plane illumination microscopy for large-scale volumetric imaging

Manish Kumar; Yevgenia Kozorovitskiy

doi:10.1364/OL.44.001706

Tilt-invariant scanned oblique plane illumination microscopy for large-scale volumetric imaging

Manish Kumar, Yevgenia Kozorovitskiy^*

^*Corresponding author for this work

Neurobiology

Research output: Contribution to journal › Article › peer-review

29 Scopus citations

Abstract

This Letter presents the first demonstration of multi-tile stitching for large scale 3D imaging in single objective light-sheet microscopy. We show undistorted 3D imaging spanning complete zebrafish larvae and over 1 mm ³ volumes for thick mouse brain sections. We use remote galvo scanning for light-sheet creation and develop a processing pipeline for 3D tiling across different axes. With the improved one photon (1p) tilt-invariant scanned oblique plane illumination (SOPi, /sōpī/) microscope presented here, we demonstrate cellular resolution imaging at depths exceeding 330 μm in optically scattering mouse brain samples and dendritic imaging in more superficial layers.

Original language	English (US)
Pages (from-to)	1706-1709
Number of pages	4
Journal	Optics Letters
Volume	44
Issue number	7
DOIs	https://doi.org/10.1364/OL.44.001706
State	Published - Apr 1 2019

Funding

Funding. National Institute of Mental Health (NIMH) (R01MH117111); Arnold and Mabel Beckman Foundation (Beckman Young Investigator Award); Kinship Foundation (Searle Scholar Award); Willliam and Bernice E. Bumpus Foundation (Young Innovator Award); Rita Allen Foundation (RAF) (Rita Allen Scholar Award); Alfred P. Sloan Foundation (Sloan Research Fellowship).

ASJC Scopus subject areas

Atomic and Molecular Physics, and Optics

Access to Document

10.1364/OL.44.001706

Cite this

@article{0bd9c43932bf407b8b1c420e35744111,

title = "Tilt-invariant scanned oblique plane illumination microscopy for large-scale volumetric imaging",

abstract = " This Letter presents the first demonstration of multi-tile stitching for large scale 3D imaging in single objective light-sheet microscopy. We show undistorted 3D imaging spanning complete zebrafish larvae and over 1 mm 3 volumes for thick mouse brain sections. We use remote galvo scanning for light-sheet creation and develop a processing pipeline for 3D tiling across different axes. With the improved one photon (1p) tilt-invariant scanned oblique plane illumination (SOPi, /sōpī/) microscope presented here, we demonstrate cellular resolution imaging at depths exceeding 330 μm in optically scattering mouse brain samples and dendritic imaging in more superficial layers.",

author = "Manish Kumar and Yevgenia Kozorovitskiy",

note = "Publisher Copyright: {\textcopyright} 2019 Optical Society of America",

year = "2019",

month = apr,

day = "1",

doi = "10.1364/OL.44.001706",

language = "English (US)",

volume = "44",

pages = "1706--1709",

journal = "Optics Letters",

issn = "0146-9592",

publisher = "Optica Publishing Group",

number = "7",

}

TY - JOUR

T1 - Tilt-invariant scanned oblique plane illumination microscopy for large-scale volumetric imaging

AU - Kumar, Manish

AU - Kozorovitskiy, Yevgenia

PY - 2019/4/1

Y1 - 2019/4/1

N2 - This Letter presents the first demonstration of multi-tile stitching for large scale 3D imaging in single objective light-sheet microscopy. We show undistorted 3D imaging spanning complete zebrafish larvae and over 1 mm 3 volumes for thick mouse brain sections. We use remote galvo scanning for light-sheet creation and develop a processing pipeline for 3D tiling across different axes. With the improved one photon (1p) tilt-invariant scanned oblique plane illumination (SOPi, /sōpī/) microscope presented here, we demonstrate cellular resolution imaging at depths exceeding 330 μm in optically scattering mouse brain samples and dendritic imaging in more superficial layers.

AB - This Letter presents the first demonstration of multi-tile stitching for large scale 3D imaging in single objective light-sheet microscopy. We show undistorted 3D imaging spanning complete zebrafish larvae and over 1 mm 3 volumes for thick mouse brain sections. We use remote galvo scanning for light-sheet creation and develop a processing pipeline for 3D tiling across different axes. With the improved one photon (1p) tilt-invariant scanned oblique plane illumination (SOPi, /sōpī/) microscope presented here, we demonstrate cellular resolution imaging at depths exceeding 330 μm in optically scattering mouse brain samples and dendritic imaging in more superficial layers.

UR - http://www.scopus.com/inward/record.url?scp=85063965230&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85063965230&partnerID=8YFLogxK

U2 - 10.1364/OL.44.001706

DO - 10.1364/OL.44.001706

M3 - Article

C2 - 30933127

AN - SCOPUS:85063965230

SN - 0146-9592

VL - 44

SP - 1706

EP - 1709

JO - Optics Letters

JF - Optics Letters

IS - 7

ER -

Tilt-invariant scanned oblique plane illumination microscopy for large-scale volumetric imaging

Abstract

Funding

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Visualization4