Tilt-invariant scanned oblique plane illumination microscopy for large-scale volumetric imaging

Manish Kumar; Yevgenia Kozorovitskiy

doi:10.1364/OL.44.001706

Tilt-invariant scanned oblique plane illumination microscopy for large-scale volumetric imaging

Manish Kumar, Yevgenia Kozorovitskiy^*

^*Corresponding author for this work

Neurobiology

Research output: Contribution to journal › Article › peer-review

20 Scopus citations

Abstract

This Letter presents the first demonstration of multi-tile stitching for large scale 3D imaging in single objective light-sheet microscopy. We show undistorted 3D imaging spanning complete zebrafish larvae and over 1 mm ³ volumes for thick mouse brain sections. We use remote galvo scanning for light-sheet creation and develop a processing pipeline for 3D tiling across different axes. With the improved one photon (1p) tilt-invariant scanned oblique plane illumination (SOPi, /sōpī/) microscope presented here, we demonstrate cellular resolution imaging at depths exceeding 330 μm in optically scattering mouse brain samples and dendritic imaging in more superficial layers.

Original language	English (US)
Pages (from-to)	1706-1709
Number of pages	4
Journal	Optics Letters
Volume	44
Issue number	7
DOIs	https://doi.org/10.1364/OL.44.001706
State	Published - Apr 1 2019

ASJC Scopus subject areas

Atomic and Molecular Physics, and Optics

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10.1364/OL.44.001706

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title = "Tilt-invariant scanned oblique plane illumination microscopy for large-scale volumetric imaging",

abstract = " This Letter presents the first demonstration of multi-tile stitching for large scale 3D imaging in single objective light-sheet microscopy. We show undistorted 3D imaging spanning complete zebrafish larvae and over 1 mm 3 volumes for thick mouse brain sections. We use remote galvo scanning for light-sheet creation and develop a processing pipeline for 3D tiling across different axes. With the improved one photon (1p) tilt-invariant scanned oblique plane illumination (SOPi, /sōpī/) microscope presented here, we demonstrate cellular resolution imaging at depths exceeding 330 μm in optically scattering mouse brain samples and dendritic imaging in more superficial layers.",

author = "Manish Kumar and Yevgenia Kozorovitskiy",

note = "Funding Information: Funding. National Institute of Mental Health (NIMH) (R01MH117111); Arnold and Mabel Beckman Foundation (Beckman Young Investigator Award); Kinship Foundation (Searle Scholar Award); Willliam and Bernice E. Bumpus Foundation (Young Innovator Award); Rita Allen Foundation (RAF) (Rita Allen Scholar Award); Alfred P. Sloan Foundation (Sloan Research Fellowship). Publisher Copyright: {\textcopyright} 2019 Optical Society of America",

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N2 - This Letter presents the first demonstration of multi-tile stitching for large scale 3D imaging in single objective light-sheet microscopy. We show undistorted 3D imaging spanning complete zebrafish larvae and over 1 mm 3 volumes for thick mouse brain sections. We use remote galvo scanning for light-sheet creation and develop a processing pipeline for 3D tiling across different axes. With the improved one photon (1p) tilt-invariant scanned oblique plane illumination (SOPi, /sōpī/) microscope presented here, we demonstrate cellular resolution imaging at depths exceeding 330 μm in optically scattering mouse brain samples and dendritic imaging in more superficial layers.

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Tilt-invariant scanned oblique plane illumination microscopy for large-scale volumetric imaging

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