Abstract
This Letter presents the first demonstration of multi-tile stitching for large scale 3D imaging in single objective light-sheet microscopy. We show undistorted 3D imaging spanning complete zebrafish larvae and over 1 mm 3 volumes for thick mouse brain sections. We use remote galvo scanning for light-sheet creation and develop a processing pipeline for 3D tiling across different axes. With the improved one photon (1p) tilt-invariant scanned oblique plane illumination (SOPi, /sōpī/) microscope presented here, we demonstrate cellular resolution imaging at depths exceeding 330 μm in optically scattering mouse brain samples and dendritic imaging in more superficial layers.
Original language | English (US) |
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Pages (from-to) | 1706-1709 |
Number of pages | 4 |
Journal | Optics Letters |
Volume | 44 |
Issue number | 7 |
DOIs | |
State | Published - Apr 1 2019 |
ASJC Scopus subject areas
- Atomic and Molecular Physics, and Optics