Tilt-invariant scanned oblique plane illumination microscopy for large-scale volumetric imaging

Manish Kumar; Yevgenia Kozorovitskiy

Tilt-invariant scanned oblique plane illumination microscopy for large-scale volumetric imaging

Manish Kumar, Yevgenia Kozorovitskiy^*

^*Corresponding author for this work

Neurobiology

Research output: Contribution to journal › Article › peer-review

Abstract

This Letter presents the first demonstration of multi-tile stitching for large scale 3D imaging in single objective light-sheet microscopy. We show undistorted 3D imaging spanning complete zebrafish larvae, and over 1 mm3 volumes for thick mouse brain sections. We use remote galvo scanning for light-sheet creation and develop a processing pipeline for 3D tiling across different axes. With the improved one photon (1p) tilt-invariant scanned oblique plane illumination (SOPi, /sopi/) microscope presented here, we demonstrate cellular resolution imaging at depths exceeding 330 μ m in optically scattering mouse brain samples, and dendritic imaging in more superficial layers.

Original language	English (US)
Journal	Unknown Journal
State	Published - Jan 2 2019

ASJC Scopus subject areas

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abstract = "This Letter presents the first demonstration of multi-tile stitching for large scale 3D imaging in single objective light-sheet microscopy. We show undistorted 3D imaging spanning complete zebrafish larvae, and over 1 mm3 volumes for thick mouse brain sections. We use remote galvo scanning for light-sheet creation and develop a processing pipeline for 3D tiling across different axes. With the improved one photon (1p) tilt-invariant scanned oblique plane illumination (SOPi, /sopi/) microscope presented here, we demonstrate cellular resolution imaging at depths exceeding 330 μ m in optically scattering mouse brain samples, and dendritic imaging in more superficial layers.",

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