Abstract
The isolated perfused rat lung model (IL) is used to study alveolar epithelial transport properties. Most of the previous studies have been done over a short period of time and have not used the same preparation as a control and intervention group. We evaluated whether the IL preparation could be used for a prolonged period of time (5 h) and studied the rates of active Na+ transport, lung liquid clearance, and passive movement of solutes. Active Na+ transport and lung liquid clearance were stable from 1 to 5 h. The passive movement of small solutes (Na+, mannitol) did not change significantly, and albumin movement increased slightly at the fifth hour. Total RNA isolated from IL after 5 h was intact, and the Na+-K+-ATPase activity in alveolar type II cells isolated at the end of 5-h experiments was equal to Na+-K+-ATPase function from freshly isolated alveolar type II cells. Finally, we measured the stimulatory effect of the β-adrenergic- agonist terbutaline and the inhibitory effect of the Na+-K+-ATPase- antagonist ouabain by using the same animal as a control. Accordingly, the isolated perfused lung model is functionally stable for at least 5 h, and it could be utilized to evaluate the effect of different interventions by using the same preparation.
Original language | English (US) |
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Pages (from-to) | 1572-1577 |
Number of pages | 6 |
Journal | Journal of applied physiology |
Volume | 85 |
Issue number | 4 |
DOIs | |
State | Published - Oct 1998 |
Keywords
- Active sodium transport
- Isolated perfused lung
- Lung edema clearance
- Lung permeability
ASJC Scopus subject areas
- Physiology (medical)
- Physiology