@article{00995c373a764800aeea27483fc48860,
title = "Tip-link integrity and mechanical transduction in vertebrate hair cells",
abstract = "An attractive hypothesis for hair-cell transduction is that fine, filamentous {"}tip links{"} pull directly on mechanically sensitive ion channels located at the tips of the stereocilia. We tested the involvement of tip links in the transduction process by treating bundles with a BAPTAbuffered, low-Ca2+ saline (10-9 M). BAPTA abolished the transduction current in a few hundred milliseconds. BAPTA treatment for a few seconds eliminated the tip links observed by either scanning or transmission electron microscopy. BAPTA also eliminated the voltagedependent movement and caused a positive bundle displacement of 133 nm, in quantitative agreement with a model for regulation of tension. We conclude that tip links convey tension to the transduction channels of hair cells.",
author = "Assad, {John A.} and Shepherd, {Gordon M G} and Corey, {David P.}",
note = "Funding Information: We thank Drs. Bruce Bean, Steven Block, and Jonathon Howard for comments on the manuscript, Robin Pinto and Karen Rock for electron microscopy, and Susan Cronin for laboratoryadmin-istration. We are especially indebted to Dr. Bechara Kachar and Marianne Parakkal for assistance with transmission electron microscopy and use of their microscope facility at the National Institutes of Health, for the experiment in Figure 5. This work was supported by grants from the NIH (DC-00304) and the Office of Naval Research (NOO14-91-J-1159), by the Howard Hughes Medical Institute, and by an NSF predoctoral fellowship to J. A. A. D. P. C. is an Associate Investigator of the Howard Hughes Medical Institute, and J. A. A and G. M. G. S. are Ryan Fellows at Harvard Medical School.",
year = "1991",
month = dec,
doi = "10.1016/0896-6273(91)90343-X",
language = "English (US)",
volume = "7",
pages = "985--994",
journal = "Neuron",
issn = "0896-6273",
publisher = "Cell Press",
number = "6",
}