Tissue biomarkers of drug efficacy: Case studies using a MALDI-MSI workflow

Elizabeth Ellen Jones; Peng Gao; Charles D. Smith; James S. Norris; Richard R. Drake

doi:10.4155/bio.15.174

Tissue biomarkers of drug efficacy: Case studies using a MALDI-MSI workflow

Elizabeth Ellen Jones, Peng Gao, Charles D. Smith, James S. Norris, Richard R. Drake^*

^*Corresponding author for this work

Medicine, Pulmonary Division

Research output: Contribution to journal › Article › peer-review

8 Scopus citations

Abstract

MALDI MS imaging (MALDI-MSI) offers a capability to not only evaluate the distribution, localization and metabolism of drugs within tissues but also allow correlative tissue measurement of the effect of the drug on biomolecules in the targeted pathway. Particularly for MALDI-MSI, lipid molecules are readily detectable within tissues. Case study examples are provided for two different drugs targeting the sphingosine-1-phosphate/ceramide nexus in tumor xenograft tissues. A workflow combining high-resolution MALDI-MSI with on-tissue confirmation of targeted compounds using a structural library and on-tissue enzymatic digestion strategy is described. Representative images of drug metabolite distribution that correlate to an increase or decrease in sphingosine-1-phosphate or ceramide species are provided.

Original language	English (US)
Pages (from-to)	2611-2619
Number of pages	9
Journal	Bioanalysis
Volume	7
Issue number	20
DOIs	https://doi.org/10.4155/bio.15.174
State	Published - Oct 2015

ASJC Scopus subject areas

Analytical Chemistry
General Pharmacology, Toxicology and Pharmaceutics
Medical Laboratory Technology
Clinical Biochemistry

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title = "Tissue biomarkers of drug efficacy: Case studies using a MALDI-MSI workflow",

abstract = "MALDI MS imaging (MALDI-MSI) offers a capability to not only evaluate the distribution, localization and metabolism of drugs within tissues but also allow correlative tissue measurement of the effect of the drug on biomolecules in the targeted pathway. Particularly for MALDI-MSI, lipid molecules are readily detectable within tissues. Case study examples are provided for two different drugs targeting the sphingosine-1-phosphate/ceramide nexus in tumor xenograft tissues. A workflow combining high-resolution MALDI-MSI with on-tissue confirmation of targeted compounds using a structural library and on-tissue enzymatic digestion strategy is described. Representative images of drug metabolite distribution that correlate to an increase or decrease in sphingosine-1-phosphate or ceramide species are provided.",

author = "Jones, {Elizabeth Ellen} and Peng Gao and Smith, {Charles D.} and Norris, {James S.} and Drake, {Richard R.}",

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AU - Jones, Elizabeth Ellen

AU - Gao, Peng

AU - Smith, Charles D.

AU - Norris, James S.

AU - Drake, Richard R.

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Tissue biomarkers of drug efficacy: Case studies using a MALDI-MSI workflow

Abstract

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