Tissue culture of epithelial cells from esophageal specialized columnar epithelium (Barrett's esophagus)

Harinder S. Garewal; Al Leibovitz; Richard E. Sampliner; Lois Ramsey; Mary J.C. Hendrix; Donna Sloan

doi:10.1007/BF01307576

Tissue culture of epithelial cells from esophageal specialized columnar epithelium (Barrett's esophagus)

Harinder S. Garewal^*, Al Leibovitz, Richard E. Sampliner, Lois Ramsey, Mary J.C. Hendrix, Donna Sloan

^*Corresponding author for this work

Robert H. Lurie Comprehensive Cancer Center

Research output: Contribution to journal › Article › peer-review

8 Scopus citations

Abstract

A procedure is described for the tissue culture of epithelial cells from Barrett's specialized columnar epithelium. The method employs use of nonenzymatic disaggregation techniques and use of a specialized growth medium, M-19. Successful growth can be achieved in 60-70% of cases. Characteristics of four cultures are presented including PAS, Alcian blue, and cytokeratin staining properties. Ultrastructural studies showed the presence of distended rough endoplasmic reticulum, abundant Golgi apparatus, glycogen granules, and cell-cell junctional complexes. Colony formation in anchorage-independent growth in soft agar was not observed in any culture. The cultured cells were not capable of indefinite growth. Thus, they do not behave as fully transformed cells. Availability of this culture system should be of use to the study of the biology of this metaplastic premalignant lesion.

Original language	English (US)
Pages (from-to)	532-536
Number of pages	5
Journal	Digestive diseases and sciences
Volume	37
Issue number	4
DOIs	https://doi.org/10.1007/BF01307576
State	Published - Apr 1992

Keywords

Barrett's esophagus
premalignant lesion
tissue culture

ASJC Scopus subject areas

Physiology
Gastroenterology

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10.1007/BF01307576

Cite this

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title = "Tissue culture of epithelial cells from esophageal specialized columnar epithelium (Barrett's esophagus)",

abstract = "A procedure is described for the tissue culture of epithelial cells from Barrett's specialized columnar epithelium. The method employs use of nonenzymatic disaggregation techniques and use of a specialized growth medium, M-19. Successful growth can be achieved in 60-70% of cases. Characteristics of four cultures are presented including PAS, Alcian blue, and cytokeratin staining properties. Ultrastructural studies showed the presence of distended rough endoplasmic reticulum, abundant Golgi apparatus, glycogen granules, and cell-cell junctional complexes. Colony formation in anchorage-independent growth in soft agar was not observed in any culture. The cultured cells were not capable of indefinite growth. Thus, they do not behave as fully transformed cells. Availability of this culture system should be of use to the study of the biology of this metaplastic premalignant lesion.",

keywords = "Barrett's esophagus, premalignant lesion, tissue culture",

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AU - Garewal, Harinder S.

AU - Leibovitz, Al

AU - Sampliner, Richard E.

AU - Ramsey, Lois

AU - Hendrix, Mary J.C.

AU - Sloan, Donna

PY - 1992/4

Y1 - 1992/4

N2 - A procedure is described for the tissue culture of epithelial cells from Barrett's specialized columnar epithelium. The method employs use of nonenzymatic disaggregation techniques and use of a specialized growth medium, M-19. Successful growth can be achieved in 60-70% of cases. Characteristics of four cultures are presented including PAS, Alcian blue, and cytokeratin staining properties. Ultrastructural studies showed the presence of distended rough endoplasmic reticulum, abundant Golgi apparatus, glycogen granules, and cell-cell junctional complexes. Colony formation in anchorage-independent growth in soft agar was not observed in any culture. The cultured cells were not capable of indefinite growth. Thus, they do not behave as fully transformed cells. Availability of this culture system should be of use to the study of the biology of this metaplastic premalignant lesion.

AB - A procedure is described for the tissue culture of epithelial cells from Barrett's specialized columnar epithelium. The method employs use of nonenzymatic disaggregation techniques and use of a specialized growth medium, M-19. Successful growth can be achieved in 60-70% of cases. Characteristics of four cultures are presented including PAS, Alcian blue, and cytokeratin staining properties. Ultrastructural studies showed the presence of distended rough endoplasmic reticulum, abundant Golgi apparatus, glycogen granules, and cell-cell junctional complexes. Colony formation in anchorage-independent growth in soft agar was not observed in any culture. The cultured cells were not capable of indefinite growth. Thus, they do not behave as fully transformed cells. Availability of this culture system should be of use to the study of the biology of this metaplastic premalignant lesion.

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KW - premalignant lesion

KW - tissue culture

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Tissue culture of epithelial cells from esophageal specialized columnar epithelium (Barrett's esophagus)

Abstract

Keywords

ASJC Scopus subject areas

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