Titanium dioxide nanoparticles assembled by DNA molecules hybridization and loading of DNA interacting proteins

Aiguo Wu, Tatjana Paunesku, Eric M.B. Brown, Angela Babbo, Cecille Cruz, Mohammed Aslam, Vinayak Dravid, Gayle E. Woloschak*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

19 Scopus citations

Abstract

This work demonstrates the assembly of TiO2 nanoparticles with attached DNA oligonucleotides into a 3D mesh structure by allowing base pairing between oligonucleotides. A change of the ratio of DNA oligonucleotide molecules and TiO2 nanoparticles regulates the size of the mesh as characterized by UV-visible light spectra, transmission electron microscopy (TEM) and atomic force microscopy (AFM) images. This type of 3D mesh, based on TiO2-DNA oligonucleotide nanoconjugates, can be used for studies of nanoparticle assemblies in materials science, energy science related to dye-sensitized solar cells, environmental science as well as characterization of DNA interacting proteins in the field of molecular biology. As an example of one such assembly, proliferating cell nuclear antigen protein (PCNA) was cloned, its activity was verified, and the protein was purified, loaded onto double strand DNA oligonucleotide- TiO2 nanoconjugates, and imaged by atomic force microscopy. This type of approach may be used to sample and perhaps quantify and/or extract specific cellular proteins from complex cellular protein mixtures based on their affinity for chosen DNA segments assembled into the 3D matrix.

Original languageEnglish (US)
Pages (from-to)27-36
Number of pages10
JournalNano
Volume3
Issue number1
DOIs
StatePublished - Feb 2008

Keywords

  • Agarose
  • Assembly
  • Atomic force microscopy
  • DNA
  • Nanoconjugate
  • PCNA protein
  • Titanium dioxide nanoparticles
  • Transmission electron microscopy

ASJC Scopus subject areas

  • Materials Science(all)
  • Condensed Matter Physics

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