Titanium dioxide nanoparticles assembled by DNA molecules hybridization and loading of DNA interacting proteins

Aiguo Wu; Tatjana Paunesku; Eric M.B. Brown; Angela Babbo; Cecille Cruz; Mohammed Aslam; Vinayak Dravid; Gayle E. Woloschak

doi:10.1142/S1793292008000836

Titanium dioxide nanoparticles assembled by DNA molecules hybridization and loading of DNA interacting proteins

Aiguo Wu, Tatjana Paunesku, Eric M.B. Brown, Angela Babbo, Cecille Cruz, Mohammed Aslam, Vinayak Dravid, Gayle E. Woloschak^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

20 Scopus citations

Abstract

This work demonstrates the assembly of TiO₂ nanoparticles with attached DNA oligonucleotides into a 3D mesh structure by allowing base pairing between oligonucleotides. A change of the ratio of DNA oligonucleotide molecules and TiO₂ nanoparticles regulates the size of the mesh as characterized by UV-visible light spectra, transmission electron microscopy (TEM) and atomic force microscopy (AFM) images. This type of 3D mesh, based on TiO₂-DNA oligonucleotide nanoconjugates, can be used for studies of nanoparticle assemblies in materials science, energy science related to dye-sensitized solar cells, environmental science as well as characterization of DNA interacting proteins in the field of molecular biology. As an example of one such assembly, proliferating cell nuclear antigen protein (PCNA) was cloned, its activity was verified, and the protein was purified, loaded onto double strand DNA oligonucleotide- TiO₂ nanoconjugates, and imaged by atomic force microscopy. This type of approach may be used to sample and perhaps quantify and/or extract specific cellular proteins from complex cellular protein mixtures based on their affinity for chosen DNA segments assembled into the 3D matrix.

Original language	English (US)
Pages (from-to)	27-36
Number of pages	10
Journal	Nano
Volume	3
Issue number	1
DOIs	https://doi.org/10.1142/S1793292008000836
State	Published - Feb 2008

Funding

This work was supported by NIH grants CA107467, EB002100, U54CA63920, and P50 CA89018. We also thank both Cell Imaging Facility and NUANCE center of Northwestern University for the use of TEM and AFM equipment and Mr. Lennell Reynolds of Cell Imaging Facility for resin embedding and sectioning of agarose embedded samples.

Keywords

Agarose
Assembly
Atomic force microscopy
DNA
Nanoconjugate
PCNA protein
Titanium dioxide nanoparticles
Transmission electron microscopy

ASJC Scopus subject areas

Condensed Matter Physics
General Materials Science

Access to Document

10.1142/S1793292008000836

Cite this

@article{1a3b957087a744a69ef204c9be4e6926,

title = "Titanium dioxide nanoparticles assembled by DNA molecules hybridization and loading of DNA interacting proteins",

abstract = "This work demonstrates the assembly of TiO2 nanoparticles with attached DNA oligonucleotides into a 3D mesh structure by allowing base pairing between oligonucleotides. A change of the ratio of DNA oligonucleotide molecules and TiO2 nanoparticles regulates the size of the mesh as characterized by UV-visible light spectra, transmission electron microscopy (TEM) and atomic force microscopy (AFM) images. This type of 3D mesh, based on TiO2-DNA oligonucleotide nanoconjugates, can be used for studies of nanoparticle assemblies in materials science, energy science related to dye-sensitized solar cells, environmental science as well as characterization of DNA interacting proteins in the field of molecular biology. As an example of one such assembly, proliferating cell nuclear antigen protein (PCNA) was cloned, its activity was verified, and the protein was purified, loaded onto double strand DNA oligonucleotide- TiO2 nanoconjugates, and imaged by atomic force microscopy. This type of approach may be used to sample and perhaps quantify and/or extract specific cellular proteins from complex cellular protein mixtures based on their affinity for chosen DNA segments assembled into the 3D matrix.",

keywords = "Agarose, Assembly, Atomic force microscopy, DNA, Nanoconjugate, PCNA protein, Titanium dioxide nanoparticles, Transmission electron microscopy",

author = "Aiguo Wu and Tatjana Paunesku and Brown, {Eric M.B.} and Angela Babbo and Cecille Cruz and Mohammed Aslam and Vinayak Dravid and Woloschak, {Gayle E.}",

note = "Funding Information: This work was supported by NIH grants CA107467, EB002100, U54CA63920, and P50 CA89018. We also thank both Cell Imaging Facility and NUANCE center of Northwestern University for the use of TEM and AFM equipment and Mr. Lennell Reynolds of Cell Imaging Facility for resin embedding and sectioning of agarose embedded samples.",

year = "2008",

month = feb,

doi = "10.1142/S1793292008000836",

language = "English (US)",

volume = "3",

pages = "27--36",

journal = "Nano",

issn = "1793-2920",

publisher = "World Scientific Publishing Co. Pte Ltd",

number = "1",

}

TY - JOUR

T1 - Titanium dioxide nanoparticles assembled by DNA molecules hybridization and loading of DNA interacting proteins

AU - Wu, Aiguo

AU - Paunesku, Tatjana

AU - Brown, Eric M.B.

AU - Babbo, Angela

AU - Cruz, Cecille

AU - Aslam, Mohammed

AU - Dravid, Vinayak

AU - Woloschak, Gayle E.

N1 - Funding Information: This work was supported by NIH grants CA107467, EB002100, U54CA63920, and P50 CA89018. We also thank both Cell Imaging Facility and NUANCE center of Northwestern University for the use of TEM and AFM equipment and Mr. Lennell Reynolds of Cell Imaging Facility for resin embedding and sectioning of agarose embedded samples.

PY - 2008/2

Y1 - 2008/2

N2 - This work demonstrates the assembly of TiO2 nanoparticles with attached DNA oligonucleotides into a 3D mesh structure by allowing base pairing between oligonucleotides. A change of the ratio of DNA oligonucleotide molecules and TiO2 nanoparticles regulates the size of the mesh as characterized by UV-visible light spectra, transmission electron microscopy (TEM) and atomic force microscopy (AFM) images. This type of 3D mesh, based on TiO2-DNA oligonucleotide nanoconjugates, can be used for studies of nanoparticle assemblies in materials science, energy science related to dye-sensitized solar cells, environmental science as well as characterization of DNA interacting proteins in the field of molecular biology. As an example of one such assembly, proliferating cell nuclear antigen protein (PCNA) was cloned, its activity was verified, and the protein was purified, loaded onto double strand DNA oligonucleotide- TiO2 nanoconjugates, and imaged by atomic force microscopy. This type of approach may be used to sample and perhaps quantify and/or extract specific cellular proteins from complex cellular protein mixtures based on their affinity for chosen DNA segments assembled into the 3D matrix.

AB - This work demonstrates the assembly of TiO2 nanoparticles with attached DNA oligonucleotides into a 3D mesh structure by allowing base pairing between oligonucleotides. A change of the ratio of DNA oligonucleotide molecules and TiO2 nanoparticles regulates the size of the mesh as characterized by UV-visible light spectra, transmission electron microscopy (TEM) and atomic force microscopy (AFM) images. This type of 3D mesh, based on TiO2-DNA oligonucleotide nanoconjugates, can be used for studies of nanoparticle assemblies in materials science, energy science related to dye-sensitized solar cells, environmental science as well as characterization of DNA interacting proteins in the field of molecular biology. As an example of one such assembly, proliferating cell nuclear antigen protein (PCNA) was cloned, its activity was verified, and the protein was purified, loaded onto double strand DNA oligonucleotide- TiO2 nanoconjugates, and imaged by atomic force microscopy. This type of approach may be used to sample and perhaps quantify and/or extract specific cellular proteins from complex cellular protein mixtures based on their affinity for chosen DNA segments assembled into the 3D matrix.

KW - Agarose

KW - Assembly

KW - Atomic force microscopy

KW - DNA

KW - Nanoconjugate

KW - PCNA protein

KW - Titanium dioxide nanoparticles

KW - Transmission electron microscopy

UR - http://www.scopus.com/inward/record.url?scp=41749114524&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=41749114524&partnerID=8YFLogxK

U2 - 10.1142/S1793292008000836

DO - 10.1142/S1793292008000836

M3 - Article

C2 - 19890457

AN - SCOPUS:41749114524

SN - 1793-2920

VL - 3

SP - 27

EP - 36

JO - Nano

JF - Nano

IS - 1

ER -

Titanium dioxide nanoparticles assembled by DNA molecules hybridization and loading of DNA interacting proteins

Abstract

Funding

Keywords

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this