Toolbox for In Vivo Imaging of Host–Parasite Interactions at Multiple Scales

Mariana De Niz; Florentin Spadin; Matthias Marti; Jens V. Stein; Martin Frenz; Friedrich Frischknecht

doi:10.1016/j.pt.2019.01.002

Toolbox for In Vivo Imaging of Host–Parasite Interactions at Multiple Scales

Mariana De Niz^*, Florentin Spadin, Matthias Marti, Jens V. Stein, Martin Frenz, Friedrich Frischknecht

^*Corresponding author for this work

Cell & Developmental Biology

Research output: Contribution to journal › Review article › peer-review

11 Scopus citations

Abstract

Animal models have for long been pivotal for parasitology research. Over the last few years, techniques such as intravital, optoacoustic and magnetic resonance imaging, optical projection tomography, and selective plane illumination microscopy developed promising potential for gaining insights into host–pathogen interactions by allowing different visualization forms in vivo and ex vivo. Advances including increased resolution, penetration depth, and acquisition speed, together with more complex image analysis methods, facilitate tackling biological problems previously impossible to study and/or quantify. Here we discuss advances and challenges in the in vivo imaging toolbox, which hold promising potential for the field of parasitology.

Original language	English (US)
Pages (from-to)	193-212
Number of pages	20
Journal	Trends in Parasitology
Volume	35
Issue number	3
DOIs	https://doi.org/10.1016/j.pt.2019.01.002
State	Published - Mar 2019

Funding

We are grateful to Volker Heussler (IZB, University of Bern) for helpful advice and intellectual input on the techniques relevant to OPT, LSFM, and OAI, and training and discussions key to these methods, and IVM. We thank also the MIC at the University of Bern, for providing access and training in all relevant imaging methods hereby discussed. We thank Federica Moalli, Renzo Danuser (TKI, Bern); Emmanuel G. Reynaud (University College, Dublin) and Jessica Kehrer (Centre for Infectious Diseases, Heidelberg University Hospital) for helpful input for OPT and LSFM techniques and discussions. We thank Robert Nuster (IAP, Bern) for experimental help during the establishment of OAI for experimental cerebral malaria imaging. We are grateful to Angelika Hoffmann (Department of Neuroradiology, Heidelberg University Hospital) for her helpful input on MRI techniques, for kindly providing Figure 4C (MRI of a Plasmodium-infected brain), and for carefully proofreading this manuscript's sections relevant to MRI. MDN is funded by EMBO fellowship ALTF 1048-2016, and by EVIMalaR (242095 FP7) and RSHTM (000529) fellowships with Volker Heussler (IZB, Bern) related to OPT and OAI studies, as well as Swiss National Science Foundation fellowships 310030_159519 and 316030_145013 funding work carried out in his laboratory. FS and MF acknowledge the financial support of the Swiss National Science Foundation (No: 205320_179038/1). Work in the laboratory of FF is funded by a Human Frontier Science Program grant (RGY 0066) and the German Science Foundation (SFB 1129). We apologize to the many colleagues for not citing their work due to restrictions by the journal and the broad scope of the review. We are grateful to Volker Heussler (IZB, University of Bern) for helpful advice and intellectual input on the techniques relevant to OPT, LSFM, and OAI, and training and discussions key to these methods, and IVM. We thank also the MIC at the University of Bern, for providing access and training in all relevant imaging methods hereby discussed. We thank Federica Moalli, Renzo Danuser (TKI, Bern); Emmanuel G. Reynaud (University College, Dublin) and Jessica Kehrer (Centre for Infectious Diseases, Heidelberg University Hospital) for helpful input for OPT and LSFM techniques and discussions. We thank Robert Nuster (IAP, Bern) for experimental help during the establishment of OAI for experimental cerebral malaria imaging. We are grateful to Angelika Hoffmann (Department of Neuroradiology, Heidelberg University Hospital) for her helpful input on MRI techniques, for kindly providing Figure 4 C (MRI of a Plasmodium-infected brain), and for carefully proofreading this manuscript\u2019s sections relevant to MRI. MDN is funded by EMBO fellowship ALTF 1048-2016, and by EVIMalaR ( 242095 FP7) and RSHTM ( 000529 ) fellowships with Volker Heussler (IZB, Bern) related to OPT and OAI studies, as well as Swiss National Science Foundation fellowships 310030_159519 and 316030_145013 funding work carried out in his laboratory. FS and MF acknowledge the financial support of the Swiss National Science Foundation (No: 205320_179038/1). Work in the laboratory of FF is funded by a Human Frontier Science Program grant (RGY 0066) and the German Science Foundation (SFB 1129). We apologize to the many colleagues for not citing their work due to restrictions by the journal and the broad scope of the review.

Keywords

animal models
imaging
in vivo
parasitology

ASJC Scopus subject areas

Parasitology
Infectious Diseases

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.pt.2019.01.002

Cite this

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title = "Toolbox for In Vivo Imaging of Host–Parasite Interactions at Multiple Scales",

abstract = "Animal models have for long been pivotal for parasitology research. Over the last few years, techniques such as intravital, optoacoustic and magnetic resonance imaging, optical projection tomography, and selective plane illumination microscopy developed promising potential for gaining insights into host–pathogen interactions by allowing different visualization forms in vivo and ex vivo. Advances including increased resolution, penetration depth, and acquisition speed, together with more complex image analysis methods, facilitate tackling biological problems previously impossible to study and/or quantify. Here we discuss advances and challenges in the in vivo imaging toolbox, which hold promising potential for the field of parasitology.",

keywords = "animal models, imaging, in vivo, parasitology",

author = "{De Niz}, Mariana and Florentin Spadin and Matthias Marti and Stein, {Jens V.} and Martin Frenz and Friedrich Frischknecht",

note = "Funding Information: We are grateful to Volker Heussler (IZB, University of Bern) for helpful advice and intellectual input on the techniques relevant to OPT, LSFM, and OAI, and training and discussions key to these methods, and IVM. We thank also the MIC at the University of Bern, for providing access and training in all relevant imaging methods hereby discussed. We thank Federica Moalli, Renzo Danuser (TKI, Bern); Emmanuel G. Reynaud (University College, Dublin) and Jessica Kehrer (Centre for Infectious Diseases, Heidelberg University Hospital) for helpful input for OPT and LSFM techniques and discussions. We thank Robert Nuster (IAP, Bern) for experimental help during the establishment of OAI for experimental cerebral malaria imaging. We are grateful to Angelika Hoffmann (Department of Neuroradiology, Heidelberg University Hospital) for her helpful input on MRI techniques, for kindly providing Figure 4C (MRI of a Plasmodium-infected brain), and for carefully proofreading this manuscript's sections relevant to MRI. MDN is funded by EMBO fellowship ALTF 1048-2016, and by EVIMalaR (242095 FP7) and RSHTM (000529) fellowships with Volker Heussler (IZB, Bern) related to OPT and OAI studies, as well as Swiss National Science Foundation fellowships 310030_159519 and 316030_145013 funding work carried out in his laboratory. FS and MF acknowledge the financial support of the Swiss National Science Foundation (No: 205320_179038/1). Work in the laboratory of FF is funded by a Human Frontier Science Program grant (RGY 0066) and the German Science Foundation (SFB 1129). We apologize to the many colleagues for not citing their work due to restrictions by the journal and the broad scope of the review. Funding Information: We are grateful to Volker Heussler (IZB, University of Bern) for helpful advice and intellectual input on the techniques relevant to OPT, LSFM, and OAI, and training and discussions key to these methods, and IVM. We thank also the MIC at the University of Bern, for providing access and training in all relevant imaging methods hereby discussed. We thank Federica Moalli, Renzo Danuser (TKI, Bern); Emmanuel G. Reynaud (University College, Dublin) and Jessica Kehrer (Centre for Infectious Diseases, Heidelberg University Hospital) for helpful input for OPT and LSFM techniques and discussions. We thank Robert Nuster (IAP, Bern) for experimental help during the establishment of OAI for experimental cerebral malaria imaging. We are grateful to Angelika Hoffmann (Department of Neuroradiology, Heidelberg University Hospital) for her helpful input on MRI techniques, for kindly providing Figure 4 C (MRI of a Plasmodium-infected brain), and for carefully proofreading this manuscript{\textquoteright}s sections relevant to MRI. MDN is funded by EMBO fellowship ALTF 1048-2016, and by EVIMalaR ( 242095 FP7) and RSHTM ( 000529 ) fellowships with Volker Heussler (IZB, Bern) related to OPT and OAI studies, as well as Swiss National Science Foundation fellowships 310030_159519 and 316030_145013 funding work carried out in his laboratory. FS and MF acknowledge the financial support of the Swiss National Science Foundation (No: 205320_179038/1). Work in the laboratory of FF is funded by a Human Frontier Science Program grant (RGY 0066) and the German Science Foundation (SFB 1129). We apologize to the many colleagues for not citing their work due to restrictions by the journal and the broad scope of the review. Publisher Copyright: {\textcopyright} 2019 Elsevier Ltd",

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AU - De Niz, Mariana

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AU - Stein, Jens V.

AU - Frenz, Martin

AU - Frischknecht, Friedrich

N1 - Funding Information: We are grateful to Volker Heussler (IZB, University of Bern) for helpful advice and intellectual input on the techniques relevant to OPT, LSFM, and OAI, and training and discussions key to these methods, and IVM. We thank also the MIC at the University of Bern, for providing access and training in all relevant imaging methods hereby discussed. We thank Federica Moalli, Renzo Danuser (TKI, Bern); Emmanuel G. Reynaud (University College, Dublin) and Jessica Kehrer (Centre for Infectious Diseases, Heidelberg University Hospital) for helpful input for OPT and LSFM techniques and discussions. We thank Robert Nuster (IAP, Bern) for experimental help during the establishment of OAI for experimental cerebral malaria imaging. We are grateful to Angelika Hoffmann (Department of Neuroradiology, Heidelberg University Hospital) for her helpful input on MRI techniques, for kindly providing Figure 4C (MRI of a Plasmodium-infected brain), and for carefully proofreading this manuscript's sections relevant to MRI. MDN is funded by EMBO fellowship ALTF 1048-2016, and by EVIMalaR (242095 FP7) and RSHTM (000529) fellowships with Volker Heussler (IZB, Bern) related to OPT and OAI studies, as well as Swiss National Science Foundation fellowships 310030_159519 and 316030_145013 funding work carried out in his laboratory. FS and MF acknowledge the financial support of the Swiss National Science Foundation (No: 205320_179038/1). Work in the laboratory of FF is funded by a Human Frontier Science Program grant (RGY 0066) and the German Science Foundation (SFB 1129). We apologize to the many colleagues for not citing their work due to restrictions by the journal and the broad scope of the review. Funding Information: We are grateful to Volker Heussler (IZB, University of Bern) for helpful advice and intellectual input on the techniques relevant to OPT, LSFM, and OAI, and training and discussions key to these methods, and IVM. We thank also the MIC at the University of Bern, for providing access and training in all relevant imaging methods hereby discussed. We thank Federica Moalli, Renzo Danuser (TKI, Bern); Emmanuel G. Reynaud (University College, Dublin) and Jessica Kehrer (Centre for Infectious Diseases, Heidelberg University Hospital) for helpful input for OPT and LSFM techniques and discussions. We thank Robert Nuster (IAP, Bern) for experimental help during the establishment of OAI for experimental cerebral malaria imaging. We are grateful to Angelika Hoffmann (Department of Neuroradiology, Heidelberg University Hospital) for her helpful input on MRI techniques, for kindly providing Figure 4 C (MRI of a Plasmodium-infected brain), and for carefully proofreading this manuscript’s sections relevant to MRI. MDN is funded by EMBO fellowship ALTF 1048-2016, and by EVIMalaR ( 242095 FP7) and RSHTM ( 000529 ) fellowships with Volker Heussler (IZB, Bern) related to OPT and OAI studies, as well as Swiss National Science Foundation fellowships 310030_159519 and 316030_145013 funding work carried out in his laboratory. FS and MF acknowledge the financial support of the Swiss National Science Foundation (No: 205320_179038/1). Work in the laboratory of FF is funded by a Human Frontier Science Program grant (RGY 0066) and the German Science Foundation (SFB 1129). We apologize to the many colleagues for not citing their work due to restrictions by the journal and the broad scope of the review. Publisher Copyright: © 2019 Elsevier Ltd

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Toolbox for In Vivo Imaging of Host–Parasite Interactions at Multiple Scales

Abstract

Funding

Keywords

ASJC Scopus subject areas

UN SDGs

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