Abstract
Ozonated oil increases the healing of chronic diabetic wounds, but the underlying mechanisms remain unclear. We investigated the effect of topical ozonated oil on wound healing in mice with diabetes with diet-induced obesity and further elucidated the role of EGFR and IGF1R signaling in diabetic wound healing. We found that topical ozonated oil accelerated wound healing; increased phosphorylation of IGF1R, EGFR, and VEGFR; and improved vascularization at the wound leading edge in mice with diabetes with diet-induced obesity. Exposure of normal epidermal keratinocytes to ozonated medium (20 μM for 2 hours daily) increased cell proliferation and migration distance by increasing phosphorylation of IGF1R and EGFR and downstream phosphoinositide 3-kinase, protein kinase B, and extracellular signal–regulated kinase. These findings shed light on the mechanism for topical ozone action in chronic wounds and support its potential therapeutic application.
Original language | English (US) |
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Pages (from-to) | 2507-2514.e6 |
Journal | Journal of Investigative Dermatology |
Volume | 143 |
Issue number | 12 |
DOIs | |
State | Published - Dec 2023 |
Funding
Ozonated oil was a gift from Hunan Health Care Technology (Changsha, China). This work was supported by the New Xiangya Talent Projects of the Third Xiangya Hospital of Central South University (20170309) (JYL), National Institute of Arthritis and Musculoskeletal and Skin Diseases R01AR44619 and R01AR068375 (ASP), and the Northwestern Skin Biology and Diseases Resource-based Center ( P30AR075049 ) (ASP). The authors also thank Robert Lavker for his advice and review of the manuscript. Ozonated oil was a gift from Hunan Health Care Technology (Changsha, China). This work was supported by the New Xiangya Talent Projects of the Third Xiangya Hospital of Central South University (20170309) (JYL), National Institute of Arthritis and Musculoskeletal and Skin Diseases R01AR44619 and R01AR068375 (ASP), and the Northwestern Skin Biology and Diseases Resource-based Center (P30AR075049) (ASP). The authors also thank Robert Lavker for his advice and review of the manuscript. Investigation, Formal Analysis, Writing - Original Draft Preparation: YL, XQW; Conceptualization: JYL, XQW, ZBF, LHG, HM; Data Curation: JYL, XQW, YPX; Software: JYL, YYJ, DW; Funding Acquisition, Supervision and Writing - Review and Editing: ASP.
ASJC Scopus subject areas
- Biochemistry
- Molecular Biology
- Dermatology
- Cell Biology