Topographic mapping of VMH → arcuate nucleus microcircuits and their reorganization by fasting

Scott M. Sternson, Gordon M G Shepherd, Jeffrey M. Friedman*

*Corresponding author for this work

Research output: Contribution to journalArticle

208 Scopus citations

Abstract

In the hypothalamic arcuate nucleus (ARC), pro-opiomelanocortin (POMC) neurons inhibit feeding and neuropeptide-Y (NPY) neurons stimulate feeding. We tested whether neurons in the ventromedial hypothalamic nucleus (VMH), a known satiety center, activate anorexigenic neuronal pathways in the ARC by projecting either excitatory synaptic inputs to POMC neurons and/or inhibitory inputs to NPY neurons. Using laser scanning photostimulation in brain slices from transgenic mice, we found that POMC and NPY neurons, which are interspersed in the ARC, are nevertheless regulated by anatomically distinct synaptic inputs. POMC neurons received strong excitatory input from the medial VMH (mVMH), whereas NPY neurons did not and, instead, received weak inhibitory input only from within the ARC. The strength of the excitatory input from the mVMH to POMC neurons was diminished by fasting. These data identify a new molecularly defined circuit that is dynamically regulated by nutritional state in a manner consistent with the known role of the VMH as a satiety center.

Original languageEnglish (US)
Pages (from-to)1356-1363
Number of pages8
JournalNature Neuroscience
Volume8
Issue number10
DOIs
StatePublished - Oct 1 2005

ASJC Scopus subject areas

  • Neuroscience(all)

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