Towards a 3D culture of mouse ovarian follicles

Martina Belli, Giulia Vigone, Valeria Merico, Carlo Alberto Redi, Maurizio Zuccotti*, Silvia Garagna

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

34 Scopus citations


The ovarian follicle has a three-dimensional (3D) structure in which the oocyte is surrounded by tightly connected follicle cells that mediate the action of external signals and nourish the gamete during its maturation. Thus, the maintenance of follicle organization during the whole growth process is crucial for the correct acquisition of developmental competence. In recent years, much attention has been given to in vitro culture systems capable of maintaining follicle architecture. With the aim of providing a quick reference guide, in this review we will summarize the techniques developed for the 3D culture of mouse follicles.

Original languageEnglish (US)
Pages (from-to)931-937
Number of pages7
JournalInternational Journal of Developmental Biology
Issue number10-12
StatePublished - 2012
Externally publishedYes


  • 3D culture
  • Follicle architecture
  • Follicle organization
  • Matrix
  • Ovarian follicle

ASJC Scopus subject areas

  • Embryology
  • Developmental Biology


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