Tracking the dynamics of circulating tumour cell phenotypes using nanoparticle-mediated magnetic ranking

Mahla Poudineh; Peter M. Aldridge; Sharif Ahmed; Brenda J. Green; Leyla Kermanshah; Vivian Nguyen; Carmen Tu; Reza M. Mohamadi; Robert K. Nam; Aaron Hansen; Srikala S. Sridhar; Antonio Finelli; Neil E. Fleshner; Anthony M. Joshua; Edward H. Sargent; Shana O. Kelley

doi:10.1038/nnano.2016.239

Tracking the dynamics of circulating tumour cell phenotypes using nanoparticle-mediated magnetic ranking

Mahla Poudineh, Peter M. Aldridge, Sharif Ahmed, Brenda J. Green, Leyla Kermanshah, Vivian Nguyen, Carmen Tu, Reza M. Mohamadi, Robert K. Nam, Aaron Hansen, Srikala S. Sridhar, Antonio Finelli, Neil E. Fleshner, Anthony M. Joshua, Edward H. Sargent, Shana O. Kelley

Chemistry

Research output: Contribution to journal › Article › peer-review

217 Scopus citations

Abstract

Profiling the heterogeneous phenotypes of rare circulating tumour cells (CTCs) in whole blood is critical to unravelling the complex and dynamic properties of these potential clinical markers. This task is challenging because these cells are present at parts per billion levels among normal blood cells. Here we report a new nanoparticle-enabled method for CTC characterization, called magnetic ranking cytometry, which profiles CTCs on the basis of their surface expression phenotype. We achieve this using a microfluidic chip that successfully processes whole blood samples. The approach classifies CTCs with single-cell resolution in accordance with their expression of phenotypic surface markers, which is read out using magnetic nanoparticles. We deploy this new technique to reveal the dynamic phenotypes of CTCs in unprocessed blood from mice as a function of tumour growth and aggressiveness. We also test magnetic ranking cytometry using blood samples collected from cancer patients.

Original language	English (US)
Pages (from-to)	274-281
Number of pages	8
Journal	Nature nanotechnology
Volume	12
Issue number	3
DOIs	https://doi.org/10.1038/nnano.2016.239
State	Published - Mar 7 2017

ASJC Scopus subject areas

Bioengineering
Atomic and Molecular Physics, and Optics
Biomedical Engineering
General Materials Science
Condensed Matter Physics
Electrical and Electronic Engineering

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1038/nnano.2016.239

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Poudineh, M., Aldridge, P. M., Ahmed, S., Green, B. J., Kermanshah, L., Nguyen, V., Tu, C., Mohamadi, R. M., Nam, R. K., Hansen, A., Sridhar, S. S., Finelli, A., Fleshner, N. E., Joshua, A. M., Sargent, E. H., & Kelley, S. O. (2017). Tracking the dynamics of circulating tumour cell phenotypes using nanoparticle-mediated magnetic ranking. Nature nanotechnology, 12(3), 274-281. https://doi.org/10.1038/nnano.2016.239

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title = "Tracking the dynamics of circulating tumour cell phenotypes using nanoparticle-mediated magnetic ranking",

abstract = "Profiling the heterogeneous phenotypes of rare circulating tumour cells (CTCs) in whole blood is critical to unravelling the complex and dynamic properties of these potential clinical markers. This task is challenging because these cells are present at parts per billion levels among normal blood cells. Here we report a new nanoparticle-enabled method for CTC characterization, called magnetic ranking cytometry, which profiles CTCs on the basis of their surface expression phenotype. We achieve this using a microfluidic chip that successfully processes whole blood samples. The approach classifies CTCs with single-cell resolution in accordance with their expression of phenotypic surface markers, which is read out using magnetic nanoparticles. We deploy this new technique to reveal the dynamic phenotypes of CTCs in unprocessed blood from mice as a function of tumour growth and aggressiveness. We also test magnetic ranking cytometry using blood samples collected from cancer patients.",

author = "Mahla Poudineh and Aldridge, {Peter M.} and Sharif Ahmed and Green, {Brenda J.} and Leyla Kermanshah and Vivian Nguyen and Carmen Tu and Mohamadi, {Reza M.} and Nam, {Robert K.} and Aaron Hansen and Sridhar, {Srikala S.} and Antonio Finelli and Fleshner, {Neil E.} and Joshua, {Anthony M.} and Sargent, {Edward H.} and Kelley, {Shana O.}",

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Poudineh, M, Aldridge, PM, Ahmed, S, Green, BJ, Kermanshah, L, Nguyen, V, Tu, C, Mohamadi, RM, Nam, RK, Hansen, A, Sridhar, SS, Finelli, A, Fleshner, NE, Joshua, AM, Sargent, EH & Kelley, SO 2017, 'Tracking the dynamics of circulating tumour cell phenotypes using nanoparticle-mediated magnetic ranking', Nature nanotechnology, vol. 12, no. 3, pp. 274-281. https://doi.org/10.1038/nnano.2016.239

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AU - Poudineh, Mahla

AU - Aldridge, Peter M.

AU - Ahmed, Sharif

AU - Green, Brenda J.

AU - Kermanshah, Leyla

AU - Nguyen, Vivian

AU - Tu, Carmen

AU - Mohamadi, Reza M.

AU - Nam, Robert K.

AU - Hansen, Aaron

AU - Sridhar, Srikala S.

AU - Finelli, Antonio

AU - Fleshner, Neil E.

AU - Joshua, Anthony M.

AU - Sargent, Edward H.

AU - Kelley, Shana O.

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AB - Profiling the heterogeneous phenotypes of rare circulating tumour cells (CTCs) in whole blood is critical to unravelling the complex and dynamic properties of these potential clinical markers. This task is challenging because these cells are present at parts per billion levels among normal blood cells. Here we report a new nanoparticle-enabled method for CTC characterization, called magnetic ranking cytometry, which profiles CTCs on the basis of their surface expression phenotype. We achieve this using a microfluidic chip that successfully processes whole blood samples. The approach classifies CTCs with single-cell resolution in accordance with their expression of phenotypic surface markers, which is read out using magnetic nanoparticles. We deploy this new technique to reveal the dynamic phenotypes of CTCs in unprocessed blood from mice as a function of tumour growth and aggressiveness. We also test magnetic ranking cytometry using blood samples collected from cancer patients.

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Tracking the dynamics of circulating tumour cell phenotypes using nanoparticle-mediated magnetic ranking

Abstract

ASJC Scopus subject areas

UN SDGs

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