Transcript map and comparative analysis of the 1.5-Mb commonly deleted segment of human 5q31 in malignant myeloid diseases with a del(5q)

Fang Lai, Lucy A. Godley, John Joslin, Anthony A. Fernald, Jin Liu, Rafael Espinosa, Nanding Zhao, Leslie Pamintuan, Brian G. Till, Richard A. Larson, Zhijian Qian, Michelle M. Le Beau

Research output: Contribution to journalArticlepeer-review

86 Scopus citations

Abstract

Loss of a whole chromosome 5, or a del(5q), are recurring abnormalities in malignant myeloid diseases. In previous studies, we defined a commonly deleted segment (CDS) of 1.5 Mb between D5S479 and D5S500 in patients with a del(5q), and we established a P1 artificial chromosome-based contig encompassing this interval. To identify candidate tumor suppressor genes (TSGs), we developed a transcript map of the CDS. The map contains 18 genes and 12 expressed sequence tags/UniGenes. Among the 18 genes are 10 genes that were previously cloned and 8 novel genes. The newly identified genes include CDC23, which encodes a component of the anaphase-promoting complex; RAB6KIFL, which encodes a kinesin-like protein involved in organelle transport; and KLHL3, which encodes a human homologue of the Drosophila ring canal protein, kelch. We determined the intron/exon organization of 14 genes and eliminated each gene as a classical TSG by mutation analysis. In addition, we established a single-nucleotide polymorphism map as well as a map of the mouse genome that is syntenic to the CDS of human 5q31. The development of a transcription map will facilitate the molecular cloning of a myeloid leukemia suppressor gene on 5q.

Original languageEnglish (US)
Pages (from-to)235-245
Number of pages11
JournalGenomics
Volume71
Issue number2
DOIs
StatePublished - Jan 15 2001

Funding

ASJC Scopus subject areas

  • Genetics

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