Transcription Factor Binding to a DNA Zip Code Controls Interchromosomal Clustering at the Nuclear Periphery

Donna Garvey Brickner, Sara Ahmed, Lauren Meldi, Abbey Thompson, Will Light, Matthew Young, Taylor L. Hickman, Feixia Chu, Emmanuelle Fabre, Jason H. Brickner*

*Corresponding author for this work

Research output: Contribution to journalArticle

62 Scopus citations

Abstract

Active genes in yeast can be targeted to the nuclear periphery through interaction of cis-acting "DNA zip codes" with the nuclear pore complex. We find that genes with identical zip codes cluster together. This clustering was specific; pairs of genes that were targeted to the nuclear periphery by different zip codes did not cluster together. Insertion of two different zip codes (GRS I or GRS III) at an ectopic site induced clustering with endogenous genes that have that zip code. Targeting to the nuclear periphery and interaction with the nuclear pore is a prerequisite for gene clustering, but clustering can be maintained in the nucleoplasm. Finally, we find that the Put3 transcription factor recognizes the GRS I zip code to mediate both targeting to the NPC and interchromosomal clustering. These results suggest that zip-code-mediated clustering of genes at the nuclear periphery influences the three-dimensional arrangement of the yeast genome.

Original languageEnglish (US)
Pages (from-to)1234-1246
Number of pages13
JournalDevelopmental Cell
Volume22
Issue number6
DOIs
StatePublished - Jun 12 2012

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ASJC Scopus subject areas

  • Molecular Biology
  • Biochemistry, Genetics and Molecular Biology(all)
  • Developmental Biology
  • Cell Biology

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