Transcription factor NF-κB signals antianoikic function of trefoil factor 3 on intestinal epithelial cells

Yi-Hua Chen*, Yu Lu, Isabelle De Plaen, Li Ya Wang, Xiaodi Tan

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

72 Scopus citations

Abstract

Transcription factor NF-κB has both pro-apoptotic and anti-apoptotic properties depending on the cell type. Its role in the intestinal epithelial cell has not been well elucidated. Trefoil factor 3 (TFF3) is an antiapoptotic peptide secreted by intestinal goblet cells. Here we show that TFF3 activated NF-κB p50/p65 heterodimer within 1 h in IEC-18 cells (a nontransformed rat intestinal epithelial cell line). Moreover, we found that TFF3-treated IEC-18 cells are resistant to anoikis, an anchorage-related apoptosis in epithelium. In addition, the stable expression of a mutant form of the endogenous NF-κB inhibitor (IκBα(mut)) in IEC-18 cells results in a significant attenuation of anti-anoikic effect of TFF3. Taken together, these data indicate that (1) TFF3 is an endogenous gastrointestinal peptide with anti-anoikic property; (2) TFF3 activates NF-κB in enterocytes; and (3) TFF3-induced resistance to anoikis in intestinal epithelial cells is mediated by a distinct signaling cascade linked to NF-κB. Furthermore, our study implicates NF-κB as an important regulator in survival pathway of intestinal epithelial cells. (C) 2000 Academic Press.

Original languageEnglish (US)
Pages (from-to)576-582
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume274
Issue number3
DOIs
StatePublished - Aug 11 2000

Keywords

  • Anoikis
  • Apoptosis
  • Intestinal epithelium
  • Trefoil factors

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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