Transcriptional activation of human GM3 synthase (hST3Gal V) gene by valproic acid in ARPE-19 human retinal pigment epithelial cells

Naree Song, Seok Jo Kim, Haw Young Kwon, Sung Wook Son, Kyoung Sook Kim, Hee Bae Ahn, Young Choon Lee*

*Corresponding author for this work

Research output: Contribution to journalArticle

8 Scopus citations

Abstract

The present study demonstrated that valproic acid (VPA) transcriptionally regulates human GM3 synthase (hST3Gal V), which catalyzes ganglioside GM3 biosynthesis in ARPE-19 human retinal pigment epithelial cells. For this, we characterized the promoter region of the hST3Gal V gene. Functional analysis of the 5'-flanking region of the hST3Gal V gene revealed that the -177 to -83 region functions as the VPA-inducible promoter and that the CREB/ATF binding site at -143 is crucial for VPA-induced expression of hST3Gal V in ARPE-19 cells. In addition, the transcriptional activity of hST3Gal V induced by VPA in ARPE-19 cells was inhibited by SP600125, a c-Jun N-terminal kinase (JNK) inhibitor. In summary, our results identified the core promoter region in the hST3Gal V promoter and for the first time demonstrated that ATF2 binding to the CREB/ATF binding site at -143 is essential for transcriptional activation of hST3Gal V in VPA-induced ARPE-19 cells.

Original languageEnglish (US)
Pages (from-to)405-409
Number of pages5
JournalBMB Reports
Volume44
Issue number6
DOIs
StatePublished - Jun 1 2011

Keywords

  • ARPE-19
  • Ganglioside
  • Human GM3 synthase
  • Transcriptional regulation
  • Valproic acid

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology

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