Transcriptome analysis in whole blood reveals increased microbial diversity in schizophrenia

Loes M. Olde Loohuis; Serghei Mangul; Anil P.S. Ori; Guillaume Jospin; David Koslicki; Harry Taegyun Yang; Timothy Wu; Marco P. Boks; Catherine Lomen-Hoerth; Martina Wiedau-Pazos; Rita M. Cantor; Willem M. De Vos; René S. Kahn; Eleazar Eskin; Roel A. Ophoff

doi:10.1038/s41398-018-0107-9

Transcriptome analysis in whole blood reveals increased microbial diversity in schizophrenia

Loes M. Olde Loohuis, Serghei Mangul, Anil P.S. Ori, Guillaume Jospin, David Koslicki, Harry Taegyun Yang, Timothy Wu, Marco P. Boks, Catherine Lomen-Hoerth, Martina Wiedau-Pazos, Rita M. Cantor, Willem M. De Vos, René S. Kahn, Eleazar Eskin, Roel A. Ophoff^*

^*Corresponding author for this work

Surgery, Thoracic Surgery Division

Research output: Contribution to journal › Article › peer-review

73 Scopus citations

Abstract

The role of the human microbiome in health and disease is increasingly appreciated. We studied the composition of microbial communities present in blood across 192 individuals, including healthy controls and patients with three disorders affecting the brain: schizophrenia, amyotrophic lateral sclerosis, and bipolar disorder. By using high-quality unmapped RNA sequencing reads as candidate microbial reads, we performed profiling of microbial transcripts detected in whole blood. We were able to detect a wide range of bacterial and archaeal phyla in blood. Interestingly, we observed an increased microbial diversity in schizophrenia patients compared to the three other groups. We replicated this finding in an independent schizophrenia case-control cohort. This increased diversity is inversely correlated with estimated cell abundance of a subpopulation of CD8⁺ memory T cells in healthy controls, supporting a link between microbial products found in blood, immunity and schizophrenia.

Original language	English (US)
Article number	96
Journal	Translational psychiatry
Volume	8
Issue number	1
DOIs	https://doi.org/10.1038/s41398-018-0107-9
State	Published - Dec 1 2018

Funding

This work is supported by NIH/NIMH R01 5R01MH090553 (BP samples), 5R01NS058980 (ALS samples) and R01MH078075 (SCZ, Control samples), R21MH098035 (replication sample) awarded to R.A.O. L.M.O.L was financially supported by the National Institute of Neurological Disorders And Stroke of the National Institutes of Health under Award Number T32NS048004. SM acknowledges support from a QCB Collaboratory Postdoctoral Fellowship, and the QCB Collaboratory community directed by Matteo Pellegrini. SM and EE are supported by National Science Foundation grants 0513612, 0731455, 0729049, 0916676, 1065276, 1302448, 1320589 and 1331176, and National Institutes of Health grants K25-HL080079, U01-DA024417, P01-HL30568, P01-HL28481, R01-GM083198, R01-ES021801, R01-MH101782 and R01-ES022282. The authors thank Dr. Jonathan Eisen for helpful discussions and insights throughout the course of this project. We thank Dr. Lana Martin for helpful edits to the manuscript. Finally, we thank the study subjects for their participation.

ASJC Scopus subject areas

Psychiatry and Mental health
Cellular and Molecular Neuroscience
Biological Psychiatry

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Olde Loohuis, L. M., Mangul, S., Ori, A. P. S., Jospin, G., Koslicki, D., Yang, H. T., Wu, T., Boks, M. P., Lomen-Hoerth, C., Wiedau-Pazos, M., Cantor, R. M., De Vos, W. M., Kahn, R. S., Eskin, E., & Ophoff, R. A. (2018). Transcriptome analysis in whole blood reveals increased microbial diversity in schizophrenia. Translational psychiatry, 8(1), Article 96. https://doi.org/10.1038/s41398-018-0107-9

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AU - Yang, Harry Taegyun

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AU - Boks, Marco P.

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AU - Wiedau-Pazos, Martina

AU - Cantor, Rita M.

AU - De Vos, Willem M.

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AU - Ophoff, Roel A.

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Transcriptome analysis in whole blood reveals increased microbial diversity in schizophrenia

Abstract

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ASJC Scopus subject areas

UN SDGs

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