Transfection of Folate-Polylysine DNA Complexes: Evidence for Lysosomal Delivery

Kimberly A. Mislick, John D. Baldeschwieler*, Jon F. Kayyem, Thomas J. Meade

*Corresponding author for this work

Research output: Contribution to journalArticle

125 Scopus citations

Abstract

We are utilizing the folate receptor for the intracellular delivery of DNA. In this study, a folate-poly-L-lysine (FPLL) conjugate was synthesized and equilibrated with plasmid DNA encoding the firefly luciferase gene. The FPLL-DNA complexes were added to KB cells treated with chloroquine. Luciferase activity of cells incubated with FPLL-DNA was 6-fold higher than of cells exposed to poly-L-lysine (PLL)-DNA. The addition of free folic acid competitively inhibited the enhancement of gene expression. Removal of chloroquine from the media significantly inhibited transfection efficiency of FPLL-DNA complexes. We conclude that FPLL-DNA complexes are delivered into KB cells via folate receptor-mediated endocytosis and likely follow a lysosomal pathway into the cytoplasm.

Original languageEnglish (US)
Pages (from-to)512-515
Number of pages4
JournalBioconjugate Chemistry
Volume6
Issue number5
DOIs
StatePublished - 1995

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Biomedical Engineering
  • Pharmacology
  • Pharmaceutical Science
  • Organic Chemistry

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