Transfer of T-DNA and Vir proteins to plant cells by Agrobacterium tumefaciens induces expression of host genes involved in mediating transformation and suppresses host defense gene expression

Veena, Hongmei Jiang, R. W. Doerge, Stanton B. Gelvin*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

116 Scopus citations

Abstract

Agrobacterium tumefaciens is a plant pathogen that incites crown gall tumors by transferring to and expressing a portion of a resident plasmid in plant cells. Currently, little is known about the host response to Agrobacterium infection. Using suppressive subtractive hybridization and DNA macroarrays, we identified numerous plant genes that are differentially expressed during early stages of Agrobacterium-mediated transformation. Expression profiling indicates that Agrobacterium infection induces plant genes necessary for the transformation process while simultaneously repressing host defense response genes, thus indicating successful utilization of existing host cellular machinery for genetic transformation purposes. A comparison of plant responses to different strains of Agrobacterium indicates that transfer of both T-DNA and Vir proteins modulates the expression of host genes during the transformation process.

Original languageEnglish (US)
Pages (from-to)219-236
Number of pages18
JournalPlant Journal
Volume35
Issue number2
DOIs
StatePublished - Jul 2003

Keywords

  • Agrobacterium
  • BY-2 cell suspension cultures
  • Expression profiling
  • Host response
  • Macroarrays
  • Transformation

ASJC Scopus subject areas

  • Genetics
  • Plant Science
  • Cell Biology

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