Transformation of rat liver epithelial cells with v-h-ras or v-raf causes expression of mdr-1, glutathione-s-transferase-p and increased resistance to cytotoxic chemicals

Richard K. Burt*, Susan Garfield, Kay Johnson, Snorri S. Thorgeirsson

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

133 Scopus citations

Abstract

We have examined the relationship between transformation and multidrug resistance by employing the v-H-ras or v-raf oncogenes to transform rat liver epithelial (RLE) cells in vitro. The dataindicate that transformation of RLE cells with v-H-ras or v-raf results in increased resistance to the cytotoxins adriamycin, vinblastine and 2-acetylaminofluorene. This multidrug resistance is accompanied by increasing expression of P-glycoprotein (MDR-1) and glutathione-S transferase P (GST-P). Thus, neoplastic transformation ofRLE cells with v-raf or v-H-ras, independently of chemical exposure, results in multidrug resistance.

Original languageEnglish (US)
Pages (from-to)2329-2332
Number of pages4
JournalCarcinogenesis
Volume9
Issue number12
DOIs
StatePublished - Dec 1 1988

ASJC Scopus subject areas

  • Cancer Research

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