TY - JOUR
T1 - Transforming growth factor-beta (TGFβ) is chemotactic for human monocytes and induces their expression of angiogenic activity
AU - Wiseman, David M.
AU - Polverini, Peter J.
AU - Kamp, David W.
AU - Leibovich, S. Joseph
N1 - Funding Information:
This work was supported by NIH Grants GM-29135 (SJL), HL-39924 (PJP) and the award of a Welcome Foundation Travelling Scholarship toDavid Wiseman. We thank Dr. Mark Dunn for his helpful discussions and the Phlebotomists of Northwestern Memorial Hospital for their helpin blood collection. We thank Dr. Michael Sporn of the NIH for the gift of TGF/3 and Dr. Michael Shepard of Genentech for the gift of the eDNA probe for human TNFt~.
PY - 1988/12/15
Y1 - 1988/12/15
N2 - TGFβ stimulates human blood monocyte migration, with peak migratory response occurring consistently at a concentration of 16-100fg/ml. Checkerboard analysis revealed both chemotactic and chemokinetic components to this response. At higher concentrations (10-100pg/ml), TGFβ stimulated expression of angiogenic activity by monocytes. While mRNA for TNFα was undetectable in resting monocytes, high steady state levels of TNFα mRNA were rapidy induced in TGFβ-treated monocytes. TGFβ is secreted by a number of neoplastic cells as well as normal cells such as platelets and lymphocytes. TGFβ may recruit monocytes from the circulation, and subsequently activate them to express angiogenic activities such as TNFα, thus playing an important role in wound repair, inflammation and tumor growth.
AB - TGFβ stimulates human blood monocyte migration, with peak migratory response occurring consistently at a concentration of 16-100fg/ml. Checkerboard analysis revealed both chemotactic and chemokinetic components to this response. At higher concentrations (10-100pg/ml), TGFβ stimulated expression of angiogenic activity by monocytes. While mRNA for TNFα was undetectable in resting monocytes, high steady state levels of TNFα mRNA were rapidy induced in TGFβ-treated monocytes. TGFβ is secreted by a number of neoplastic cells as well as normal cells such as platelets and lymphocytes. TGFβ may recruit monocytes from the circulation, and subsequently activate them to express angiogenic activities such as TNFα, thus playing an important role in wound repair, inflammation and tumor growth.
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U2 - 10.1016/S0006-291X(88)80319-X
DO - 10.1016/S0006-291X(88)80319-X
M3 - Article
C2 - 2462419
AN - SCOPUS:0024212247
SN - 0006-291X
VL - 157
SP - 793
EP - 800
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 2
ER -