Transgenic technology: An overview of approaches useful in surgical research

D. R. Brenin, M. S. Talamonti*, P. M. Iannaccone

*Corresponding author for this work

Research output: Contribution to journalReview articlepeer-review

3 Scopus citations

Abstract

Advances in transgenic science have created powerful tools for the investigation of both genetic and protein regulatory systems. Recently, transgenic animals have been utilized in several vascular and transplantation research laboratories. The ability to specifically mutate genes important in oncologic and cardiovascular research is leading to a greater understanding of the role of gene and protein regulatory systems in cancer and cardiovascular disease. The expanding use of transgenic animals will undoubtedly increase our insight into complex problems in surgical research. This review briefly describes the various techniques utilized to create transgenic animals including: transgene design, gene-transfer utilizing transfection, microinjection and retroviral infection, as well as the use of embryonic stem cells, and methods for screening transgenic offspring.

Original languageEnglish (US)
Pages (from-to)99-110
Number of pages12
JournalSurgical Oncology
Volume6
Issue number2
DOIs
StatePublished - Aug 1 1997

Keywords

  • Chimera
  • Embryonic stem cells
  • Gene-transfer
  • Pronuclear injection
  • Transgenic

ASJC Scopus subject areas

  • Surgery
  • Oncology

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