Transglutaminase-catalyzed crosslinking of the Aa and y constituent chains in fibrinogen

S. N.Prasanna Murthy, James H. Wilson, Thomas J. Lukas, Yuri Veklich, John W. Weisel, Laszlo Lorand*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

29 Scopus citations


Studies on transglutaminases usually focus on the polymerization of protein substrates by intermolecular Nε(γ-glutamyl)lysine bridges, without considering the possibility that the monomeric protein units, themselves, could also become crosslinked internally. Both types of crosslinks are produced in the reaction of fibrinogen with red cell transglutaminase. We isolated the transglutaminase-modified, mostly monomeric form (92-96%) of fibrinogen with a Nε(γ-glutamyl)lysine content of ≈1.6 moles/mole of fibrinogen. The preparation was fully clottable by thrombin, but the rates of release of fibrinopeptides and clotting times were delayed compared with control. Hybrid Aα·γ type of crosslinking, the hallmark of the reaction of the transglutaminase with fibrinogen, occurred by bridging the Aα(408-421) chain segment of the protein to that of γ(392-406). Rotary shadowed electron microscope images showed many monomers to be bent, and the crosslinks seemed to bind the otherwise flexible αC domain closer to the backbone of fibrinogen.

Original languageEnglish (US)
Pages (from-to)44-48
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Issue number1
StatePublished - Jan 4 2000

ASJC Scopus subject areas

  • General


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