Transient activation of GABAB receptors suppresses SK channel currents in substantia nigra pars compacta dopaminergic neurons

Chad M. Estep, Daniel J. Galtieri, Enrico Zampese, Joshua A. Goldberg, Lars Brichta, Paul Greengard, D. James Surmeier*

*Corresponding author for this work

Research output: Contribution to journalArticle

4 Scopus citations

Abstract

Dopaminergic (DA) neurons in the substantia nigra pars compacta (SNc) are richly innervated by GABAergic neurons. The postsynaptic effects of GABA on SNc DA neurons are mediated by a mixture of GABAA and GABAB receptors. Although activation of GABAA receptors inhibits spike generation, the consequences of GABAB receptor activation are less well characterized. To help fill this gap, perforated patch recordings were made from young adult mouse SNc DA neurons. Sustained stimulation of GABAB receptors hyperpolarized SNc DA neurons, as previously described. However, transient stimulation of GABAB receptors by optical uncaging of GABA did not; rather, it reduced the opening of small-conductance, calcium-activated K+ (SK) channels and increased the irregularity of spiking. This modulation was attributable to inhibition of adenylyl cyclase and protein kinase A. Thus, because suppression of SK channel activity increases the probability of burst spiking, transient co-activation of GABAA and GABAB receptors could promote a pause-burst pattern of spiking.

Original languageEnglish (US)
Article numbere0169044
JournalPloS one
Volume11
Issue number12
DOIs
StatePublished - Dec 2016

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)
  • General

Fingerprint Dive into the research topics of 'Transient activation of GABA<sub>B</sub> receptors suppresses SK channel currents in substantia nigra pars compacta dopaminergic neurons'. Together they form a unique fingerprint.

  • Cite this