TY - JOUR
T1 - Transient and persistent dendritic spines in the neocortex in vivo
AU - Holtmaat, Anthony J G D
AU - Trachtenberg, Joshua T.
AU - Wilbrecht, Linda
AU - Shepherd, Gordon M.
AU - Zhang, Xiaoqun
AU - Knott, Graham W.
AU - Svoboda, Karel
N1 - Funding Information:
We thank Brian Chen and Barry Burbach for help with experiments; Vincenzo De Paola, Carlos Portera, Karen Zito, and Egbert Welker for comments on the manuscript; and Josh Sanes for the GFP-M mice. This work was supported by the Netherlands Institute for Brain Research (AH); the Swiss National Foundation (GK); NIH; and HHMI.
PY - 2005/1/20
Y1 - 2005/1/20
N2 - Dendritic spines were imaged over days to months in the apical tufts of neocortical pyramidal neurons (layers 5 and 2/3) in vivo. A fraction of thin spines appeared and disappeared over a few days, while most thick spines persisted for months. In the somatosensory cortex, from postnatal day (PND) 16 to PND 25 spine retractions exceeded additions, resulting in a net loss of spines. The fraction of persistent spines (lifetime ≥ 8 days) grew gradually during development and into adulthood (PND 16-25, 35%; PND 35-80, 54%; PND 80-120, 66%; PND 175-225, 73%), providing evidence that synaptic circuits continue to stabilize even in the adult brain, long after the closure of known critical periods. In 6-month-old mice, spines turn over more slowly in visual compared to somatosensory cortex, possibly reflecting differences in the capacity for experience-dependent plasticity in these brain regions.
AB - Dendritic spines were imaged over days to months in the apical tufts of neocortical pyramidal neurons (layers 5 and 2/3) in vivo. A fraction of thin spines appeared and disappeared over a few days, while most thick spines persisted for months. In the somatosensory cortex, from postnatal day (PND) 16 to PND 25 spine retractions exceeded additions, resulting in a net loss of spines. The fraction of persistent spines (lifetime ≥ 8 days) grew gradually during development and into adulthood (PND 16-25, 35%; PND 35-80, 54%; PND 80-120, 66%; PND 175-225, 73%), providing evidence that synaptic circuits continue to stabilize even in the adult brain, long after the closure of known critical periods. In 6-month-old mice, spines turn over more slowly in visual compared to somatosensory cortex, possibly reflecting differences in the capacity for experience-dependent plasticity in these brain regions.
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U2 - 10.1016/j.neuron.2005.01.003
DO - 10.1016/j.neuron.2005.01.003
M3 - Article
C2 - 15664179
AN - SCOPUS:12344250057
VL - 45
SP - 279
EP - 291
JO - Neuron
JF - Neuron
SN - 0896-6273
IS - 2
ER -