TY - JOUR
T1 - Translational effects of differential codon usage among intragenic domains of new genes in Drosophila
AU - Zhang, Jianming
AU - Long, Manyuan
AU - Li, Liming
N1 - Funding Information:
We thank the members of Long's lab for their experimental assistance and helpful discussions, especially Ana Llopart's assistance for cloning jgw from D. teissieri, and Hong Qin, Janice Spofford, Todd Martin, and two anonymous reviewers for suggestions and discussions. This project was supported by US Army Medical Research and Material Command grant DAMD17-03-1-0282 to L.L and by a NIH R01 grant (GM65429-01A1) and a NSF CAREER award (0238168) to M.L.
PY - 2005/5/1
Y1 - 2005/5/1
N2 - Evolved codon usages often pose a technical challenge over the expressing of eukaryotic genes in microbial systems because of changed translational machinery. In the present study, we investigated the translational effects of intragenic differential codon usage on the expression of the new Drosophila gene, jingwei (jgw), a chimera derived from two unrelated parental genes: Ymp and Adh. We found that jgw possesses a strong intragenic differential usage of synonymous codons, i.e. the Adh-derived C-domain has a significantly higher codon bias than that of the Ymp-derived N-domain (P=0.0023 by t-test). Additional evolutionary analysis revealed the heterogeneous distribution of rare codons, implicating its role in gene regulation and protein translation. The in vitro expression of jgw further demonstrated that the heterogeneous distribution of rare codons has played a role in regulating gene expression, particularly, affecting the quality of protein translation.
AB - Evolved codon usages often pose a technical challenge over the expressing of eukaryotic genes in microbial systems because of changed translational machinery. In the present study, we investigated the translational effects of intragenic differential codon usage on the expression of the new Drosophila gene, jingwei (jgw), a chimera derived from two unrelated parental genes: Ymp and Adh. We found that jgw possesses a strong intragenic differential usage of synonymous codons, i.e. the Adh-derived C-domain has a significantly higher codon bias than that of the Ymp-derived N-domain (P=0.0023 by t-test). Additional evolutionary analysis revealed the heterogeneous distribution of rare codons, implicating its role in gene regulation and protein translation. The in vitro expression of jgw further demonstrated that the heterogeneous distribution of rare codons has played a role in regulating gene expression, particularly, affecting the quality of protein translation.
KW - Analysis of protein functions
KW - Codon usage
KW - Evolution of new genes
KW - Expression of insect genes
KW - Intragenic codon bias
KW - Short-chain dehydrogenase
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U2 - 10.1016/j.bbaexp.2005.02.004
DO - 10.1016/j.bbaexp.2005.02.004
M3 - Article
C2 - 15833448
AN - SCOPUS:18044378220
SN - 0167-4781
VL - 1728
SP - 135
EP - 142
JO - Biochimica et Biophysica Acta - Gene Structure and Expression
JF - Biochimica et Biophysica Acta - Gene Structure and Expression
IS - 3
ER -