TRNA residues evolved to promote translational accuracy

Irina Shepotinovskaya, Olke C. Uhlenbeck*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

11 Scopus citations


The decoding properties of 22 structurally conservative base-pair and base-triple mutations in the anticodon hairpin and tertiary core of Escherichia coli tRNAGGCAla were determined under single turnover conditions using E. coli ribosomes. While all of the mutations were able to efficiently decode the cognate GCC codon, many showed substantial misreading of near-cognate GUC or ACC codons. Although all the misreading mutations were present in the sequences of other E. coli tRNAs, they were never found among bacterial tRNAGGCAla sequences. This suggests that the sequences of bacterial tRNAGGCAla have evolved to avoid reading incorrect codons.

Original languageEnglish (US)
Pages (from-to)510-516
Number of pages7
Issue number4
StatePublished - Apr 2013


  • Protein synthesis
  • Ribosome

ASJC Scopus subject areas

  • Molecular Biology


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