tRNA residues that have coevolved with their anticodon to ensure uniform and accurate codon recognition

Mikołaj Olejniczak, Olke C Uhlenbeck*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

49 Scopus citations

Abstract

The structure, phylogeny and in vivo function of the base pair formed between nucleotides 32 and 38 of the tRNA anticodon loop are reviewed. The A32-U38 pair, which is highly conserved in tRNA2 Ala and sometimes observed in tRNA2 Pro, was recently found to decrease the affinity of tRNAs to the ribosomal A site relative to other 32-38 combinations. This suggests that the role of 32-38 pair is to tune the tRNA affinity in the A site to a uniform value. New experiments presented here show that the U32C mutation in tRNA1 Gly increases its affinity to the cognate codon and to codons with third position mismatches in the A site. This suggests that one reason for uniform tRNA binding to evolve was to avoid incorrect codon recognition.

Original languageEnglish (US)
Pages (from-to)943-950
Number of pages8
JournalBiochimie
Volume88
Issue number8
DOIs
StatePublished - Aug 1 2006

Keywords

  • Decoding
  • Translation
  • tRNA tuning
  • tRNA uniformity
  • Two-out-of-three reading

ASJC Scopus subject areas

  • Biochemistry

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