Trophoblasts Regulate the Placental Hematopoietic Niche through PDGF-B Signaling

Akanksha Chhabra, Andrew J. Lechner, Masaya Ueno, Asha Acharya, Ben Van Handel, Yanling Wang, M. Luisa Iruela-Arispe, Michelle D. Tallquist, Hanna K.A. Mikkola*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

44 Scopus citations


The placenta is a hematopoietic organ that supports hematopoietic stem/progenitor cell (HSPC) generation and expansion without promoting differentiation. We identified PDGF-B signaling in trophoblasts as a key component of the unique placental hematopoietic microenvironment that protects HSPCs from premature differentiation. Loss of PDGF-B or its receptor, PDGFRβ, induced definitive erythropoiesis in placental labyrinth vasculature. This was evidenced by accumulation of CFU-Es and actively proliferating definitive erythroblasts that clustered around central macrophages, highly reminiscent of erythropoiesis in the fetal liver. Ectopic erythropoiesis was not due to a requirement of PDGF-B signaling in hematopoietic cells but rather in placental trophoblasts, which upregulated Epo in the absence of PDGF-B signaling. Furthermore, overexpression of hEPO specifically in the trophoblasts in vivo was sufficient to convert the placenta into an erythropoietic organ. These data provide genetic evidence of a signaling pathway that is required to restrict erythroid differentiation to specific anatomical niches during development. Video Abstract: The placenta is an important embryonic niche for hematopoietic stem/progenitor cells (HSPCs). Chhabra et al. find that PDGF signaling suppresses Epo production by placental trophoblasts and thereby attenuates placental erythropoiesis. Thus, PDGF signaling determines niche characteristics that in turn influence resident HSPCs to control the cell-type specificity of HSPC output.

Original languageEnglish (US)
Pages (from-to)651-659
Number of pages9
JournalDevelopmental Cell
Issue number3
StatePublished - Mar 13 2012

ASJC Scopus subject areas

  • General Biochemistry, Genetics and Molecular Biology
  • Molecular Biology
  • Cell Biology
  • Developmental Biology


Dive into the research topics of 'Trophoblasts Regulate the Placental Hematopoietic Niche through PDGF-B Signaling'. Together they form a unique fingerprint.

Cite this