Truncated hemoglobin o of Mycobacterium tuberculosis: The oligomeric state change and the interaction with membrane components

Chong Liu, Yuan He, Zengyi Chang*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

40 Scopus citations

Abstract

Being an obligate aerobe, the Mycobacterium tuberculosis cells would have to evolve a mechanism to collect and deliver the hardly available O2 to survive in granulomas and to maintain the low level of respiration during latency. The M. tuberculosis truncated hemoglobin o (trHbO), when heterologously expressed in Escherichia coli cells, was found to significantly enhance the cellular respiration and cell growth. This study was undertaken in an attempt to understand the molecular details for trHbO to promote the cellular respiration, focusing on the ways through which trHbO is recruited to the cell membrane and O2 molecules are delivered. Our data demonstrate that the trHbO protein is able to promote the growth of E. coli cells in a fashion that depends on the presence of the respiratory chain terminal oxidase cytochrome o complex (or Cyo complex). The trHbO protein appears to interact with the Cyo B subunit of the Cyo complex directly, likely in a dynamic manner. The trHbO is also able to bind membrane lipids in a non-specific way, during the process electrostatic and hydrophobic interactions both likely exist. Besides, binding with membrane induces the dissociation of trHbO from dimers to monomers. In light of these observations, a hypothesis was made to explain how trHbO might serve as an O2 collector and/or reservoir for M. tuberculosis cells under O2-limiting or lacking conditions.

Original languageEnglish (US)
Pages (from-to)1163-1172
Number of pages10
JournalBiochemical and Biophysical Research Communications
Volume316
Issue number4
DOIs
StatePublished - Apr 16 2004

Keywords

  • Membrane binding
  • Mycobacterium tuberculosis
  • Oligomeric change
  • Oxygen delivery
  • Truncated hemoglobin o

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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