TULIPs: Tunable, light-controlled interacting protein tags for cell biology

Devin Strickland, Yuan Lin, Elizabeth Wagner, C. Matthew Hope, Josiah Zayner, Chloe Antoniou, Tobin R. Sosnick, Eric L. Weiss, Michael Glotzer*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

274 Scopus citations

Abstract

Naturally photoswitchable proteins offer a means of directly manipulating the formation of protein complexes that drive a diversity of cellular processes. We developed tunable light-inducible dimerization tags (TULIPs) based on a synthetic interaction between the LOV2 domain of Avena sativa phototropin 1 (AsLOV2) and an engineered PDZ domain (ePDZ). TULIPs can recruit proteins to diverse structures in living yeast and mammalian cells, either globally or with precise spatial control using a steerable laser. The equilibrium binding and kinetic parameters of the interaction are tunable by mutation, making TULIPs readily adaptable to signaling pathways with varying sensitivities and response times. We demonstrate the utility of TULIPs by conferring light sensitivity to functionally distinct components of the yeast mating pathway and by directing the site of cell polarization.

Original languageEnglish (US)
Pages (from-to)379-384
Number of pages6
JournalNature Methods
Volume9
Issue number4
DOIs
StatePublished - Apr 1 2012

ASJC Scopus subject areas

  • Biotechnology
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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