TY - JOUR
T1 - Tumor angiogenesis and bone metastasis - Correlation in invasive breast carcinoma
AU - Sun, Chong
AU - Li, Jianmin
AU - Wang, Bin
AU - Shangguan, Junjie
AU - Figini, Matteo
AU - Shang, Na
AU - Pan, Liang
AU - Zhang, Zhuoli
N1 - Funding Information:
This study was generously supported by NIH NCI RO1CA196967 and RO1CA209886 . Chong Sun is grateful for the State Scholarship Fund from the China Scholarship Council (Postgraduate program sponsored by government on behalf of national high level university construction).
Publisher Copyright:
© 2017 Elsevier B.V.
PY - 2018/1
Y1 - 2018/1
N2 - Purpose To test the hypotheses that pathological biomarkers including CD34 positive endothelial cell and microvessel density (MVD) of the primary breast tumor can be used to predict the probability of occurrence for bone metastases and provide information for appropriate therapeutic strategies at an early stage. Methods Three groups of CD34 immunohistochemical stained slices (n = 60) were acquired from surgical specimens of sixty patients including non-metastasis (group 1), only lymph node metastasis (group 2), and bone metastasis (group 3). MVD was measured by TissueGnostics system. Results The MVD measurement results were 57.14 ± 23.00 in group 1, 86.44 ± 21.13 in group 2, and 126.85 ± 47.89 in group 3. There were statistical differences between group 1 and group 2 (P = 0.0002), between group 2 and group 3 (P = 0.0014) and between group 1 and group 3 (P < 0.0001). The strong correlations were found between CD34 positive cell measurement and its percentage (group 1, r = 0.74, P = 0.0002; group 2, r = 0.62, P = 0.0034; group 3, r = 0.84, P < 0.0001), and between CD34 positive endothelial cell measurement and MVD (r = 0.61, P < 0.0001). Conclusions Quantitative CD34 positive endothelial cell and MVD measurements of the primary breast tumor have a strong correlation with the occurrence rate of bone metastases, which predicts the probability of occurrence for bone metastases at an early stage.
AB - Purpose To test the hypotheses that pathological biomarkers including CD34 positive endothelial cell and microvessel density (MVD) of the primary breast tumor can be used to predict the probability of occurrence for bone metastases and provide information for appropriate therapeutic strategies at an early stage. Methods Three groups of CD34 immunohistochemical stained slices (n = 60) were acquired from surgical specimens of sixty patients including non-metastasis (group 1), only lymph node metastasis (group 2), and bone metastasis (group 3). MVD was measured by TissueGnostics system. Results The MVD measurement results were 57.14 ± 23.00 in group 1, 86.44 ± 21.13 in group 2, and 126.85 ± 47.89 in group 3. There were statistical differences between group 1 and group 2 (P = 0.0002), between group 2 and group 3 (P = 0.0014) and between group 1 and group 3 (P < 0.0001). The strong correlations were found between CD34 positive cell measurement and its percentage (group 1, r = 0.74, P = 0.0002; group 2, r = 0.62, P = 0.0034; group 3, r = 0.84, P < 0.0001), and between CD34 positive endothelial cell measurement and MVD (r = 0.61, P < 0.0001). Conclusions Quantitative CD34 positive endothelial cell and MVD measurements of the primary breast tumor have a strong correlation with the occurrence rate of bone metastases, which predicts the probability of occurrence for bone metastases at an early stage.
KW - Bone metastasis
KW - Breast carcinoma
KW - Pathological biomarkers
KW - Tumor angiogenesis
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U2 - 10.1016/j.jim.2017.10.006
DO - 10.1016/j.jim.2017.10.006
M3 - Article
C2 - 29066178
AN - SCOPUS:85032359836
SN - 0022-1759
VL - 452
SP - 46
EP - 52
JO - Journal of Immunological Methods
JF - Journal of Immunological Methods
ER -