Tumor-prone phenotype of the DDB2-deficient mice

Taewon Yoon, Amit Chakrabortty, Roberta Franks, Ted Valli, Hiroaki Kiyokawa, Pradip Raychaudhuri*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

64 Scopus citations

Abstract

DDB2 is an essential subunit of the damaged-DNA recognition factor DDB, which is involved in global genomic repair in human cells. Moreover, DDB2 is mutated in the repair-deficiency disease xeroderma pigmentosum (Group E). Expression of DDB2 in human cells is induced by P53, BRCA1 and by ionizing radiation. The DDB2 protein associates with transcriptional activator and coactivator proteins. In addition, DDB2 in conjunction with DDB1 associates with cullin 4A and the Cop9/signalosome. We generated a mouse strain deficient for DDB2 (DDB2-/-). Consistent with the human disease (XP-E), the DDB2-/- mice were susceptible to UV-induced skin carcinogenesis. We observed a significant difference in the initial rate of cyclobutane pyrimidine dimer (CPD)-removal from the skin following UV irradiation. Also, the DDB2-deficient mice exhibited a significantly reduced life span compared to their wild-type littermates. Moreover, unlike other XP-deficient mice, the DDB2-deficient mice developed spontaneous malignant tumors at a high rate between the ages of 20 and 25 months. The observations suggest that, in addition to DNA repair, the other interactions of DDB2 are significant in its tumor suppression function.

Original languageEnglish (US)
Pages (from-to)469-478
Number of pages10
JournalOncogene
Volume24
Issue number3
DOIs
StatePublished - Jan 13 2005

Keywords

  • DDB2
  • DDB2 knockout mice
  • DNA repair
  • UV-induced tumor
  • XP-E

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics
  • Cancer Research

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