Tumor-suppressive mir-663 gene induces mitotic catastrophe growth arrest in human gastric cancer cells

Jian Pan, Hai Hu, Zhuan Zhou, Lichao Sun, Liang Peng, Long Yu, Lixin Sun, Jun Liu, Zhihua Yang, Yuliang Ran*

*Corresponding author for this work

Research output: Contribution to journalArticle

86 Scopus citations

Abstract

Increasing evidence suggests that microRNAs are involved in human carcinogenesis as tumor suppressors or oncogenes. A growing number of reports has shown that an interest has been sparked in aberrant microRNA expression and how they can be used to treat human diseases, including cancer. However, their precise biological role remains largely unknown. In the present study, we aimed to identify micro-RNA species involved in the regulation of tumor growth. By performing quantitative reverse transcription-polymerase chain reaction (RT-PCR) analysis, we demonstrated that mir-663 was downregulated in human gastric cancer cell lines unlike in normal cells. A transient introduction of mir-663 into the human gastric cancer cell lines BGC823 and SNU5 induced morphology changes and suppression of proliferation of these cells. In addition, mir-663 alters the DNA content and induces phenotypes of mitotic catastrophe in tumor cells. Moreover, the liposome-mediated delivery of mir-663 suppressed the in vivo growth of the BGC823 and SNU5 cells. Western blot analyses performed after the introduction of mir-663 revealed upregulation of cyclin B following transfection with mir-663. Our results provide evidence that downregulation of mir-663 in tumor cells may contribute to aberrant cell hyperplasia, leading to the development of gastric cancer. Therefore, mir-663 might function as a potent suppressor of tumor growth.

Original languageEnglish (US)
Pages (from-to)105-112
Number of pages8
JournalOncology reports
Volume24
Issue number1
DOIs
StatePublished - Jan 1 2010

Keywords

  • Gastric cancer
  • Mitotic catastrophe
  • mir-663

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

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    Pan, J., Hu, H., Zhou, Z., Sun, L., Peng, L., Yu, L., Sun, L., Liu, J., Yang, Z., & Ran, Y. (2010). Tumor-suppressive mir-663 gene induces mitotic catastrophe growth arrest in human gastric cancer cells. Oncology reports, 24(1), 105-112. https://doi.org/10.3892/or-00000834