TY - JOUR
T1 - Tuning multi/pluri-potent stem cell fate by electrospun poly(l-lactic acid)-calcium-deficient hydroxyapatite nanocomposite mats
AU - D'Angelo, Francesco
AU - Armentano, Ilaria
AU - Cacciotti, Ilaria
AU - Tiribuzi, Roberto
AU - Quattrocelli, Mattia
AU - Del Gaudio, Costantino
AU - Fortunati, Elena
AU - Saino, Enrica
AU - Caraffa, Auro
AU - Cerulli, Giuliano Giorgio
AU - Visai, Livia
AU - Kenny, Josè Maria
AU - Sampaolesi, Maurilio
AU - Bianco, Alessandra
AU - Martino, Sabata
AU - Orlacchio, Aldo
PY - 2012/5/14
Y1 - 2012/5/14
N2 - In this study, we investigated whether multipotent (human-bone-marrow- derived mesenchymal stem cells [hBM-MSCs]) and pluripotent stem cells (murine-induced pluripotent stem cells [iPSCs] and murine embryonic stem cells [ESCs]) respond to nanocomposite fibrous mats of poly(l-lactic acid) (PLLA) loaded with 1 or 8 wt % of calcium-deficient nanohydroxyapatite (d-HAp). Remarkably, the dispersion of different amounts of d-HAp to PLLA produced a set of materials (PLLA/d-HAp) with similar architectures and tunable mechanical properties. After 3 weeks of culture in the absence of soluble osteogenic factors, we observed the expression of osteogenic markers, including the deposition of bone matrix proteins, in multi/pluripotent cells only grown on PLLA/d-HAp nanocomposites, whereas the osteogenic differentiation was absent on stem-cell-neat PLLA cultures. Interestingly, this phenomenon was confined only in hBM-MSCs, murine iPSCs, and ESCs grown on direct contact with the PLLA/d-HAp mats. Altogether, these results indicate that the osteogenic differentiation effect of these electrospun PLLA/d-HAp nanocomposites was independent of the stem cell type and highlight the direct interaction of stem cell-polymeric nanocomposite and the mechanical properties acquired by the PLLA/d-HAp nanocomposites as key steps for the differentiation process.
AB - In this study, we investigated whether multipotent (human-bone-marrow- derived mesenchymal stem cells [hBM-MSCs]) and pluripotent stem cells (murine-induced pluripotent stem cells [iPSCs] and murine embryonic stem cells [ESCs]) respond to nanocomposite fibrous mats of poly(l-lactic acid) (PLLA) loaded with 1 or 8 wt % of calcium-deficient nanohydroxyapatite (d-HAp). Remarkably, the dispersion of different amounts of d-HAp to PLLA produced a set of materials (PLLA/d-HAp) with similar architectures and tunable mechanical properties. After 3 weeks of culture in the absence of soluble osteogenic factors, we observed the expression of osteogenic markers, including the deposition of bone matrix proteins, in multi/pluripotent cells only grown on PLLA/d-HAp nanocomposites, whereas the osteogenic differentiation was absent on stem-cell-neat PLLA cultures. Interestingly, this phenomenon was confined only in hBM-MSCs, murine iPSCs, and ESCs grown on direct contact with the PLLA/d-HAp mats. Altogether, these results indicate that the osteogenic differentiation effect of these electrospun PLLA/d-HAp nanocomposites was independent of the stem cell type and highlight the direct interaction of stem cell-polymeric nanocomposite and the mechanical properties acquired by the PLLA/d-HAp nanocomposites as key steps for the differentiation process.
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U2 - 10.1021/bm3000716
DO - 10.1021/bm3000716
M3 - Article
C2 - 22449037
AN - SCOPUS:84861138099
SN - 1525-7797
VL - 13
SP - 1350
EP - 1360
JO - Biomacromolecules
JF - Biomacromolecules
IS - 5
ER -