Tuning multi/pluri-potent stem cell fate by electrospun poly(l-lactic acid)-calcium-deficient hydroxyapatite nanocomposite mats

Francesco D'Angelo, Ilaria Armentano, Ilaria Cacciotti, Roberto Tiribuzi, Mattia Quattrocelli, Costantino Del Gaudio, Elena Fortunati, Enrica Saino, Auro Caraffa, Giuliano Giorgio Cerulli, Livia Visai, Josè Maria Kenny, Maurilio Sampaolesi, Alessandra Bianco, Sabata Martino*, Aldo Orlacchio

*Corresponding author for this work

Research output: Contribution to journalArticle

66 Scopus citations

Abstract

In this study, we investigated whether multipotent (human-bone-marrow- derived mesenchymal stem cells [hBM-MSCs]) and pluripotent stem cells (murine-induced pluripotent stem cells [iPSCs] and murine embryonic stem cells [ESCs]) respond to nanocomposite fibrous mats of poly(l-lactic acid) (PLLA) loaded with 1 or 8 wt % of calcium-deficient nanohydroxyapatite (d-HAp). Remarkably, the dispersion of different amounts of d-HAp to PLLA produced a set of materials (PLLA/d-HAp) with similar architectures and tunable mechanical properties. After 3 weeks of culture in the absence of soluble osteogenic factors, we observed the expression of osteogenic markers, including the deposition of bone matrix proteins, in multi/pluripotent cells only grown on PLLA/d-HAp nanocomposites, whereas the osteogenic differentiation was absent on stem-cell-neat PLLA cultures. Interestingly, this phenomenon was confined only in hBM-MSCs, murine iPSCs, and ESCs grown on direct contact with the PLLA/d-HAp mats. Altogether, these results indicate that the osteogenic differentiation effect of these electrospun PLLA/d-HAp nanocomposites was independent of the stem cell type and highlight the direct interaction of stem cell-polymeric nanocomposite and the mechanical properties acquired by the PLLA/d-HAp nanocomposites as key steps for the differentiation process.

Original languageEnglish (US)
Pages (from-to)1350-1360
Number of pages11
JournalBiomacromolecules
Volume13
Issue number5
DOIs
StatePublished - May 14 2012

ASJC Scopus subject areas

  • Bioengineering
  • Biomaterials
  • Polymers and Plastics
  • Materials Chemistry

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    D'Angelo, F., Armentano, I., Cacciotti, I., Tiribuzi, R., Quattrocelli, M., Del Gaudio, C., Fortunati, E., Saino, E., Caraffa, A., Cerulli, G. G., Visai, L., Kenny, J. M., Sampaolesi, M., Bianco, A., Martino, S., & Orlacchio, A. (2012). Tuning multi/pluri-potent stem cell fate by electrospun poly(l-lactic acid)-calcium-deficient hydroxyapatite nanocomposite mats. Biomacromolecules, 13(5), 1350-1360. https://doi.org/10.1021/bm3000716