### Abstract

Two-parameter equations that describe the dependence of lnk upon φ, where k is the retention factor and φ the volume fraction of the organic modifier in the mobile phase, are examined in what concerns the underlying approximations and their performance to fit experimental data obtained from reversed-phase liquid chromatography. Using 293 experimental systems, it was found that the performance of these equations to describe lnk versus φ data is rather low, since the percentage of the systems that can be described satisfactorily ranges from 40 to 60% depending on the fitting equation. This percentage may be raised to 75%, if the discreteness effect is properly taken into account. A further improvement to 90% of the systems studied can be achieved only by the use of three-parameter equations, which may arise by refinements of the rough approximations of the two-parameter equations. Although the refinements do not lead always to better equations, we developed a new three-parameter expression of lnk that works more satisfactorily, since it combines simplicity, linearity of its adjustable parameters and the highest applicability.

Original language | English (US) |
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Pages (from-to) | 29-41 |

Number of pages | 13 |

Journal | Journal of Chromatography A |

Volume | 1033 |

Issue number | 1 |

DOIs | |

State | Published - Apr 9 2004 |

### Keywords

- Liquid chromatography
- Mathematical modelling
- Retention models

### ASJC Scopus subject areas

- Analytical Chemistry
- Biochemistry
- Organic Chemistry

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## Cite this

*Journal of Chromatography A*,

*1033*(1), 29-41. https://doi.org/10.1016/j.chroma.2004.01.021