Two-dimensional profiling of cancer cell subpopulations

Mahla Poudineh*, Mahmoud Labib, Edward H. Sargent, Shana O. Kelley

*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

The rare cells that are released from cancerous tumors into the bloodstream have a potential to monitor cancer progression. Here, we describe a 2D microfluidic approach that can deconvolute properties of heterogeneous cell subpopulations. Cancer cells are first profiled according to their surface marker expression. In the second dimension, the subsets are further separated into 20 subpopulations, according to their migratory behaviours toward a chemotactic agent. We present a set of studies investigating the chemical gradient generation in migration channels. We show that this method can be used to profile cancer cells even in the presence of whole blood.

Original languageEnglish (US)
Title of host publication21st International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2017
PublisherChemical and Biological Microsystems Society
Pages1637-1638
Number of pages2
ISBN (Electronic)9780692941836
StatePublished - 2020
Externally publishedYes
Event21st International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2017 - Savannah, United States
Duration: Oct 22 2017Oct 26 2017

Publication series

Name21st International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2017

Conference

Conference21st International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2017
Country/TerritoryUnited States
CitySavannah
Period10/22/1710/26/17

Keywords

  • Cell sorting
  • Chemotaxis
  • Microfluidics

ASJC Scopus subject areas

  • Chemical Engineering (miscellaneous)
  • Bioengineering

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