Abstract
The rare cells that are released from cancerous tumors into the bloodstream have a potential to monitor cancer progression. Here, we describe a 2D microfluidic approach that can deconvolute properties of heterogeneous cell subpopulations. Cancer cells are first profiled according to their surface marker expression. In the second dimension, the subsets are further separated into 20 subpopulations, according to their migratory behaviours toward a chemotactic agent. We present a set of studies investigating the chemical gradient generation in migration channels. We show that this method can be used to profile cancer cells even in the presence of whole blood.
| Original language | English (US) |
|---|---|
| Title of host publication | 21st International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2017 |
| Publisher | Chemical and Biological Microsystems Society |
| Pages | 1637-1638 |
| Number of pages | 2 |
| ISBN (Electronic) | 9780692941836 |
| State | Published - 2020 |
| Event | 21st International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2017 - Savannah, United States Duration: Oct 22 2017 → Oct 26 2017 |
Publication series
| Name | 21st International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2017 |
|---|
Conference
| Conference | 21st International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2017 |
|---|---|
| Country/Territory | United States |
| City | Savannah |
| Period | 10/22/17 → 10/26/17 |
Funding
We acknowledge support from the Canadian Institutes of Health Research (Emerging Team grant, POP Grant) and the Ontario Research Fund (ORF Research Excellence grant).
Keywords
- Cell sorting
- Chemotaxis
- Microfluidics
ASJC Scopus subject areas
- Chemical Engineering (miscellaneous)
- Bioengineering
