Two distinct T cell subsets, CD4+ and CD8+ CD60 +, and their cytokines are required for in vitro induction of human Ragweed-specific memory IgE responses

Tamar A. Smith-Norowitz, Jonathan Silverberg, Kevin B. Norowitz, Martin H. Bluth, Seto Chice, Rauno Joks, Maja Nowakowski, Helen G. Durkin

Research output: Contribution to journalArticlepeer-review

4 Scopus citations

Abstract

CD8+CD60+ T cells (80-98% CD45RO+; 20% CD23+) are significantly increased in the blood of serum IgE + ragweed-sensitized (RS) compared with serum IgE-nonatopic humans (p = 0.001). CD8+CD60+ T cells of the RS patients produced IL-2, IL-4, IL-10, IL-12, IFN-α. and IFN-γ, but not IL-6 or IL-13. When their PBMC were cultured with ragweed Ag (RA), peak IgE responses occurred on day 10; none was induced with non-cross-reacting or without Ag; nonatopic PBMC did not respond to any stimulant. When either CD4+ or CD8 +CD60+ T cells were depleted from RS PBMC before culture with RA, no IgE responses were induced. If purified CD4+ T cells or low numbers of CD8+CD60+ T cells were added back to the depleted PBMC, IgE responses were restored. However, higher numbers of CD8 +CD60+ T cells totally suppressed IgE responses. Total suppression also was obtained when RS PBMC were cultured with RA and either anti-IL-2, IL-4, IL-10, IL-12, IFN-γ (all concentrations), or IFN-α (low concentrations), but not anti-IL-6 or IL-13. Higher concentrations of anti-IFN-α potentiated IgE responses.

Original languageEnglish (US)
Pages (from-to)4761-4769
Number of pages9
JournalJournal of Immunology
Volume181
Issue number7
DOIs
StatePublished - Oct 1 2008

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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