Two growth hormone receptors in Nile tilapia (Oreochromis niloticus): Molecular characterization, tissue distribution and expression profiles in the gonad during the reproductive cycle

Xilan Ma; Xiaochun Liu; Yong Zhang; Pei Zhu; Wei Ye; Haoran Lin

doi:10.1016/j.cbpb.2007.01.021

Two growth hormone receptors in Nile tilapia (Oreochromis niloticus): Molecular characterization, tissue distribution and expression profiles in the gonad during the reproductive cycle

Xilan Ma, Xiaochun Liu, Yong Zhang, Pei Zhu, Wei Ye, Haoran Lin^*

^*Corresponding author for this work

Biochemistry and Molecular Genetics

Research output: Contribution to journal › Article › peer-review

60 Scopus citations

Abstract

Growth hormone (GH) has many important physiological roles in the control of growth, metabolism and reproduction, which is mediated by growth hormone receptor (GHR). In this study, two cDNAs encoding GHR were isolated from the liver of Nile tilapia (Oreochromis niloticus). The two cDNAs, one consisting of 1908 bp and the other of 1728 bp, encoding for putative 635- and 575-amino acid GHR (designated ntGHR1 and ntGHR2, respectively), shared 34.4% identity in nucleotide sequence and 29.6% in deduced amino acid sequence. Northern blot analysis indicated a single 6.0-kb transcript of ntGHR1 and a single 4.0-kb transcript of ntGHR2 in the liver. Real-time RT-PCR analysis showed that both ntGHR1 and ntGHR2 mRNAs were presented in all tissues tested and expressed extremely highly in the liver. In most tissues, ntGHR2 expressed significantly higher than ntGHR1. Analysis of the ntGHRs expression profiles in the gonad during reproductive cycle indicated that the mRNA levels of ntGHRs in ovary were significantly higher at sexual matured stage while those in testis were significantly higher at sexual recrudescent stage, suggesting that GH/IGF-I axis might be involved in reproduction under a regulatory mechanism of GHR gene expression.

Original language	English (US)
Pages (from-to)	325-339
Number of pages	15
Journal	Comparative Biochemistry and Physiology - B Biochemistry and Molecular Biology
Volume	147
Issue number	2
DOIs	https://doi.org/10.1016/j.cbpb.2007.01.021
State	Published - Jun 2007

Funding

This research was funded by the grants from Guangdong Provincial Scientific and Technical Program (2004A20105001), Zhuhai Scientific and Technical Program (PC20051054), Guangdong Provincial Oceanic and Fishery Administration Special Fund, and Specialized Research Fund for the Doctoral Program of Higher Education (20040558012). The authors are grateful to the staff of Panyu State-level Tilapia Breeding Center and to the graduate students in our lab (Weiren Huang, Zijie Long, Yun Liu, Shuisheng Li) for their assistance.

Keywords

Growth hormone receptor
Nile tilapia (Oreochromis niloticus)
Northern blot
Real-time RT-PCR
Reproductive cycle
Tissue distribution

ASJC Scopus subject areas

Molecular Biology
Biochemistry
Physiology

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10.1016/j.cbpb.2007.01.021

Cite this

Ma, X., Liu, X., Zhang, Y., Zhu, P., Ye, W., & Lin, H. (2007). Two growth hormone receptors in Nile tilapia (Oreochromis niloticus): Molecular characterization, tissue distribution and expression profiles in the gonad during the reproductive cycle. Comparative Biochemistry and Physiology - B Biochemistry and Molecular Biology, 147(2), 325-339. https://doi.org/10.1016/j.cbpb.2007.01.021

@article{5611d7265ea34b03b2c83bf5c1227816,

title = "Two growth hormone receptors in Nile tilapia (Oreochromis niloticus): Molecular characterization, tissue distribution and expression profiles in the gonad during the reproductive cycle",

abstract = "Growth hormone (GH) has many important physiological roles in the control of growth, metabolism and reproduction, which is mediated by growth hormone receptor (GHR). In this study, two cDNAs encoding GHR were isolated from the liver of Nile tilapia (Oreochromis niloticus). The two cDNAs, one consisting of 1908 bp and the other of 1728 bp, encoding for putative 635- and 575-amino acid GHR (designated ntGHR1 and ntGHR2, respectively), shared 34.4% identity in nucleotide sequence and 29.6% in deduced amino acid sequence. Northern blot analysis indicated a single 6.0-kb transcript of ntGHR1 and a single 4.0-kb transcript of ntGHR2 in the liver. Real-time RT-PCR analysis showed that both ntGHR1 and ntGHR2 mRNAs were presented in all tissues tested and expressed extremely highly in the liver. In most tissues, ntGHR2 expressed significantly higher than ntGHR1. Analysis of the ntGHRs expression profiles in the gonad during reproductive cycle indicated that the mRNA levels of ntGHRs in ovary were significantly higher at sexual matured stage while those in testis were significantly higher at sexual recrudescent stage, suggesting that GH/IGF-I axis might be involved in reproduction under a regulatory mechanism of GHR gene expression.",

keywords = "Growth hormone receptor, Nile tilapia (Oreochromis niloticus), Northern blot, Real-time RT-PCR, Reproductive cycle, Tissue distribution",

author = "Xilan Ma and Xiaochun Liu and Yong Zhang and Pei Zhu and Wei Ye and Haoran Lin",

note = "Funding Information: This research was funded by the grants from Guangdong Provincial Scientific and Technical Program (2004A20105001), Zhuhai Scientific and Technical Program (PC20051054), Guangdong Provincial Oceanic and Fishery Administration Special Fund, and Specialized Research Fund for the Doctoral Program of Higher Education (20040558012). The authors are grateful to the staff of Panyu State-level Tilapia Breeding Center and to the graduate students in our lab (Weiren Huang, Zijie Long, Yun Liu, Shuisheng Li) for their assistance.",

year = "2007",

month = jun,

doi = "10.1016/j.cbpb.2007.01.021",

language = "English (US)",

volume = "147",

pages = "325--339",

journal = "Comparative Biochemistry and Physiology - B Biochemistry and Molecular Biology",

issn = "1096-4959",

publisher = "Elsevier Inc.",

number = "2",

}

Ma, X, Liu, X, Zhang, Y, Zhu, P, Ye, W & Lin, H 2007, 'Two growth hormone receptors in Nile tilapia (Oreochromis niloticus): Molecular characterization, tissue distribution and expression profiles in the gonad during the reproductive cycle', Comparative Biochemistry and Physiology - B Biochemistry and Molecular Biology, vol. 147, no. 2, pp. 325-339. https://doi.org/10.1016/j.cbpb.2007.01.021

Two growth hormone receptors in Nile tilapia (Oreochromis niloticus): Molecular characterization, tissue distribution and expression profiles in the gonad during the reproductive cycle. / Ma, Xilan; Liu, Xiaochun; Zhang, Yong et al.
In: Comparative Biochemistry and Physiology - B Biochemistry and Molecular Biology, Vol. 147, No. 2, 06.2007, p. 325-339.

Research output: Contribution to journal › Article › peer-review

TY - JOUR

T1 - Two growth hormone receptors in Nile tilapia (Oreochromis niloticus)

T2 - Molecular characterization, tissue distribution and expression profiles in the gonad during the reproductive cycle

AU - Ma, Xilan

AU - Liu, Xiaochun

AU - Zhang, Yong

AU - Zhu, Pei

AU - Ye, Wei

AU - Lin, Haoran

N1 - Funding Information: This research was funded by the grants from Guangdong Provincial Scientific and Technical Program (2004A20105001), Zhuhai Scientific and Technical Program (PC20051054), Guangdong Provincial Oceanic and Fishery Administration Special Fund, and Specialized Research Fund for the Doctoral Program of Higher Education (20040558012). The authors are grateful to the staff of Panyu State-level Tilapia Breeding Center and to the graduate students in our lab (Weiren Huang, Zijie Long, Yun Liu, Shuisheng Li) for their assistance.

PY - 2007/6

Y1 - 2007/6

N2 - Growth hormone (GH) has many important physiological roles in the control of growth, metabolism and reproduction, which is mediated by growth hormone receptor (GHR). In this study, two cDNAs encoding GHR were isolated from the liver of Nile tilapia (Oreochromis niloticus). The two cDNAs, one consisting of 1908 bp and the other of 1728 bp, encoding for putative 635- and 575-amino acid GHR (designated ntGHR1 and ntGHR2, respectively), shared 34.4% identity in nucleotide sequence and 29.6% in deduced amino acid sequence. Northern blot analysis indicated a single 6.0-kb transcript of ntGHR1 and a single 4.0-kb transcript of ntGHR2 in the liver. Real-time RT-PCR analysis showed that both ntGHR1 and ntGHR2 mRNAs were presented in all tissues tested and expressed extremely highly in the liver. In most tissues, ntGHR2 expressed significantly higher than ntGHR1. Analysis of the ntGHRs expression profiles in the gonad during reproductive cycle indicated that the mRNA levels of ntGHRs in ovary were significantly higher at sexual matured stage while those in testis were significantly higher at sexual recrudescent stage, suggesting that GH/IGF-I axis might be involved in reproduction under a regulatory mechanism of GHR gene expression.

AB - Growth hormone (GH) has many important physiological roles in the control of growth, metabolism and reproduction, which is mediated by growth hormone receptor (GHR). In this study, two cDNAs encoding GHR were isolated from the liver of Nile tilapia (Oreochromis niloticus). The two cDNAs, one consisting of 1908 bp and the other of 1728 bp, encoding for putative 635- and 575-amino acid GHR (designated ntGHR1 and ntGHR2, respectively), shared 34.4% identity in nucleotide sequence and 29.6% in deduced amino acid sequence. Northern blot analysis indicated a single 6.0-kb transcript of ntGHR1 and a single 4.0-kb transcript of ntGHR2 in the liver. Real-time RT-PCR analysis showed that both ntGHR1 and ntGHR2 mRNAs were presented in all tissues tested and expressed extremely highly in the liver. In most tissues, ntGHR2 expressed significantly higher than ntGHR1. Analysis of the ntGHRs expression profiles in the gonad during reproductive cycle indicated that the mRNA levels of ntGHRs in ovary were significantly higher at sexual matured stage while those in testis were significantly higher at sexual recrudescent stage, suggesting that GH/IGF-I axis might be involved in reproduction under a regulatory mechanism of GHR gene expression.

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KW - Nile tilapia (Oreochromis niloticus)

KW - Northern blot

KW - Real-time RT-PCR

KW - Reproductive cycle

KW - Tissue distribution

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Two growth hormone receptors in Nile tilapia (Oreochromis niloticus): Molecular characterization, tissue distribution and expression profiles in the gonad during the reproductive cycle

Abstract

Funding

Keywords

ASJC Scopus subject areas

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