Two mRNAs that differ by two nontemplated nucleotides encode the amino coterminal proteins P and V of the paramyxovirus SV5

Sheila M. Thomas*, Robert A. Lamb, Reay G. Paterson

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

247 Scopus citations

Abstract

The "P≓ gene of the paramyxovirus SV5 encodes two known proteins, P (Mr ≈ 44,000) and V (Mr ≈ 24,000). The complete nucleotide sequence of the "P≓ gene has been obtained and is found to contain two open reading frames, neither of which is large enough to encode the P protein. We have shown that the P and V proteins are translated from two mRNAs that differ by the presence of two nontemplated G residues in the P mRNA. These two additional nucleotides convert the two open reading frames to one of 392 amino acids. The P and V proteins are amino coterminal and have 164 amino acids in common. The unique C terminus of V consists of a cysteine-rich region that resembles a cysteine-rich metal binding domain. An open reading frame that contains this cysteine-rich region exists in all other paramyxovirus "P≓ gene sequences examined, which suggests that it may have important biological significance.

Original languageEnglish (US)
Pages (from-to)891-902
Number of pages12
JournalCell
Volume54
Issue number6
DOIs
StatePublished - Sep 9 1988

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

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